| Bone morphogenetic protein-4 enhances vascular endothelial growth factor secretion by human retinal pigment epithelial cells. | |
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MedLine Citation:
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PMID: 16514669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retinal pigment epithelial (RPE) cells secrete vascular endothelial growth factor (VEGF), a cytokine known to promote angiogenesis. Results from RNase protection assays (RPAs) show that RPE from non-diabetic human donors and from adult retinal pigment epithelium-19 (ARPE-19) cells expressed significant bone morphogenetic protein-4 (BMP-4) message. In addition, ARPE-19 cells cultured in high glucose (25 mM), compared to those in physiological glucose (5.5 mM) released significantly more BMP-4 into the conditioned media (CM). However, the effect of BMP-4 on the release of VEGF by ARPE-19 cells has not been studied. Accordingly, ARPE-19 cells were treated with BMP-4 to determine VEGF secretion. BMP-4 and VEGF levels in the CM and cell lysates were measured by enzyme-linked immunosorbent assay (ELISA). Cells treated with exogenous BMP-4 had higher VEGF in the CM and this treatment effect was dose- and time-dependent, while cell lysates had low levels of VEGF. Addition of cycloheximide (CHX) or actinomycin-D (ACT) significantly reduced VEGF secretion from cells treated with BMP-4, suggesting that the BMP-4-induced secretion of VEGF requires new RNA and protein synthesis. Our results suggest that BMP-4 may play a role in the regulation of ocular angiogenesis associated with diabetic retinopathy (DR) by stimulating VEGF release from RPE cells. |
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Authors:
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Rhonda R Vogt; Richard Unda; Lee-Chuan C Yeh; Eileen K Vidro; John C Lee; Andrew T Tsin |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 98 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-25 Completed Date: 2006-09-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1196-202 Citation Subset: IM |
Copyright Information:
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(c) 2006 Wiley-Liss, Inc. |
Affiliation:
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Department of Biology, The University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / genetics, metabolism*, pharmacology, secretion Cell Line Cycloheximide / pharmacology Dactinomycin / pharmacology Epithelial Cells / drug effects, metabolism*, secretion Glucose / pharmacology Humans Male Pigment Epithelium of Eye / drug effects, metabolism*, secretion* Retina / drug effects, metabolism*, secretion* Vascular Endothelial Growth Factor A / secretion* |
| Grant Support | |
ID/Acronym/Agency:
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GM 07761/GM/NIGMS NIH HHS; GM 08194/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/BMP4 protein, human; 0/Bone Morphogenetic Protein 4; 0/Bone Morphogenetic Proteins; 0/Vascular Endothelial Growth Factor A; 50-76-0/Dactinomycin; 50-99-7/Glucose; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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