| Bone marrow stromal cells protect myeloma cells from bortezomib induced apoptosis by suppressing microRNA-15a expression. | |
| | |
MedLine Citation:
|
PMID: 21534877 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Despite unsurpassed anti-tumor activity of bortezomib for multiple myeloma (MM), drug resistance has emerged as a challenge, especially when MM cells adhere to the stroma. This study aimed to determine whether bone marrow stromal cells (BMSCs) have a role in the development of chemoresistance in MM. Our data demonstrate that the secretion of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and cell-to-cell contact with microenvironment-derived stromal cells from patients with multiple myeloma (MM-BMSCs) significantly decreased the sensitivity of myeloma cells to bortezomib treatment. Mechanistically, we found that microRNA (miRNA)- 15a expression was up-regulated in U266 and NCI-H929 cells treated by bortezomib, which was inhibited by MM-BMSCs. miRNA-15a transfected myeloma cells were arrested in G1/S checkpoint and secreted less VEGF compared to control transfected cells, although no significant difference was found in VEGF mRNA levels. In conclusion, our data suggest that via suppressing miRNA-15a expression, BMSCs provide survival support and protect myeloma cells from bortezomib induced apoptosis. |
| | |
Authors:
|
Mu Hao; Li Zhang; Gang An; Hengxing Meng; Youjin Han; Zhenqing Xie; Yan Xu; Changhong Li; Zhen Yu; Hong Chang; Lugui Qiu |
Related Documents
:
|
16169967 - Epidermal cell death in rice is regulated by ethylene, gibberellin, and abscisic acid. 7531347 - Fas-based d10s-mediated cytotoxicity requires macromolecular synthesis for effector cel... 10585287 - Transient overexpression of murine dishevelled genes results in apoptotic cell death. 10837337 - Two distinct mechanisms of nitric oxide-mediated neuronal cell death show thiol depende... 1382917 - D-glyceraldehyde-3-phosphate dehydrogenase from hela cells--2. immunological characteri... 17571257 - Engineering cell physiology to enhance recombinant protein production in escherichia coli. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-3 |
Journal Detail:
|
Title: Leukemia & lymphoma Volume: - ISSN: 1029-2403 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prognostic impact of t(1;19)/ TCF3-PBX1 in childhood acute lymphoblastic leukemia in the context of ...
Next Document: Management and palliative chemotherapy for metastatic or recurrent squamous cell carcinoma of the he...