| Bone marrow derived pluripotent cells are pericytes which contribute to vascularization. | |
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MedLine Citation:
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PMID: 20058207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pericytes are essential to vascularization, but the purification and characterization of pericytes remain unclear. Smooth muscle actin alpha (alpha-SMA) is one marker [corrected] of pericytes. The aim of this study is to purify the alpha-SMA positive cells from bone marrow and study the characteristics of these cells and the interaction between alpha-SMA positive cells and endothelial cells. The bone marrow stromal cells were harvested from alpha-SMA-GFP transgenic mice, and the alpha-SMA-GFP positive cells were sorted by FACS. The proliferative characteristics and multilineage differentiation ability of the alpha-SMA-GFP positive cells were tested. A 3-D culture model was then applied to test their vascularization by loading alpha-SMA-GFP positive cells and endothelial cells on collagen-fibronectin gel. Results demonstrated that bone marrow stromal cells are mostly alpha-SMA-GFP positive cells which are pluripotent, and these cells expressed alpha-SMA during differentiation. The alpha-SMA-GFP positive cells could stimulate the endothelial cells to form tube-like structures and subsequently robust vascular networks in 3-D culture. In conclusion, the bone marrow derived pluripotent cells include [corrected] pericytes and can contribute to vascularization. |
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Authors:
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Xiaoxiao Cai; Yunfeng Lin; Claudia C Friedrich; Craig Neville; Irina Pomerantseva; Cathryn A Sundback; Parul Sharma; Zhiyuan Zhang; Joseph P Vacanti; Peter V Hauschka; Brian E Grottkau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Stem cell reviews Volume: 5 ISSN: 1558-6804 ISO Abbreviation: Stem Cell Rev Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-08 Completed Date: 2010-03-01 Revised Date: 2010-06-02 |
Medline Journal Info:
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Nlm Unique ID: 101255952 Medline TA: Stem Cell Rev Country: United States |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu 610041, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics,
metabolism Animals Bone Marrow Cells / cytology*, metabolism Cell Culture Techniques Cell Differentiation Cell Proliferation Cell Separation Endothelial Cells / cytology, metabolism Humans Mice Mice, Transgenic Neovascularization, Physiologic* Pluripotent Stem Cells / cytology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins |
| Comments/Corrections | |
Erratum In:
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Stem Cell Rev. 2009 Dec;5(4):435-6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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