| Bone marrow-derived cultured mast cells and peritoneal mast cells as targets of a growth activity secreted by BALB/3T3 fibroblasts. | |
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MedLine Citation:
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PMID: 1847337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When fibroblast cell lines were cultured in contact with bone marrow-derived cultured mast cells (CMC), both NIH/3T3 and BALB/3T3 cell lines supported the proliferation of CMC. In contrast, when contact between fibroblasts and CMC was prohibited by Biopore membranes or soft agar, only BALB/3T3 fibroblasts supported CMC proliferation, suggesting that BALB/3T3 but not NIH/3T3 cells secreted a significant amount of a mast cell growth activity. Moreover, the BALB/3T3-derived growth activity induced the incorporation of [3H]thymidine by CMC and the clonal growth of peritoneal mast cells in methylcellulose. The mast cell growth activity appeared to be different from interleukin 3 (IL-3) and interleukin 4 (IL-4), because mRNAs for these interleukins were not detectable in BALB/3T3 fibroblasts. Although mast cells are genetically deficient in tissues of W/Wv mice, CMC did develop when bone marrow cells of W/Wv mice were cultured with pokeweed mitogen-stimulated spleen cell-conditioned medium. Because BALB/3T3 fibroblast-conditioned medium (BALB-FCM) did not induce the incorporation of [3H]thymidine by W/Wv CMC, the growth activity in BALB-FCM appeared to be a ligand for the receptor encoded by the W (c-kit) locus. Because CMC and peritoneal mast cells are obtained as homogeneous suspensions rather easily, these cells may be potentially useful as targets for the fibroblast-derived mast cell growth activity. |
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Authors:
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K Jozaki; A Kuriu; S Hirota; H Onoue; Y Ebi; S Adachi; J Y Ma; S Tarui; Y Kitamura |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental hematology Volume: 19 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-03-26 Completed Date: 1991-03-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 185-90 Citation Subset: IM |
Affiliation:
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Department of Medicine, Osaka University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow / drug effects, ultrastructure Bone Marrow Cells* Cell Division / drug effects Cell Separation Cells, Cultured Culture Media / analysis, pharmacology Fibroblasts / chemistry, cytology*, metabolism Growth Substances / analysis, metabolism, pharmacology* Ligands Mast Cells / cytology*, drug effects, ultrastructure Mice Mice, Inbred BALB C Peritoneal Cavity / cytology* Receptors, Cell Surface / genetics, metabolism Thymidine / metabolism Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Growth Substances; 0/Ligands; 0/Receptors, Cell Surface; 10028-17-8/Tritium; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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