Document Detail


Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor.
MedLine Citation:
PMID:  2017189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.
Authors:
R G Hammonds; R Schwall; A Dudley; L Berkemeier; C Lai; J Lee; N Cunningham; A H Reddi; W I Wood; A J Mason
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  5     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-23     Completed Date:  1991-05-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  149-55     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Bone Development / drug effects*
Bone Morphogenetic Proteins
Bone and Bones / drug effects
Cell Line
Cloning, Molecular*
Gene Expression
Humans
Molecular Sequence Data
Protein Precursors / genetics*
Proteins / chemistry,  genetics*,  pharmacology
Rats
Recombinant Fusion Proteins / genetics*,  pharmacology
Transfection
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/Protein Precursors; 0/Proteins; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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