| Bone Marrow-derived Myofibroblasts Are the Providers of Pro-invasive Matrix Metalloproteinase 13 in Primary Tumor. | |
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MedLine Citation:
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PMID: 23097628 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Carcinoma-associated fibroblasts are key contributors of the tumor microenvironment that regulates carcinoma progression. They consist of a heterogeneous cell population with diverse origins, phenotypes, and functions. In the present report, we have explored the contribution of bone marrow (BM)-derived cells to generate different fibroblast subsets that putatively produce the matrix metalloproteinase 13 (MMP13) and affect cancer cell invasion. A murine model of skin carcinoma was applied to mice, irradiated, and engrafted with BM isolated from green fluorescent protein (GFP) transgenic mice. We provide evidence that one third of BM-derived GFP(+) cells infiltrating the tumor expressed the chondroitin sulfate proteoglycan NG2 (pericytic marker) or α-smooth muscle actin (α-SMA, myofibroblast marker), whereas almost 90% of Thy1(+) fibroblasts were originating from resident GFP-negative cells. MMP13producing cells were exclusively α-SMA(+) cells and derived from GFP(+) BM cells. To investigate their impact on tumor invasion, we isolated mesenchymal stem cells (MSCs) from the BM of wild-type and MMP13-deficient mice. Wild-type MSC promoted cancer cell invasion in a spheroid assay, whereas MSCs obtained from MMP13-deficient mice failed to. Our data support the concept of fibroblast subset specialization with BM-derived α-SMA(+) cells being the main source of MMP13, a stromal mediator of cancer cell invasion. |
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Authors:
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Julie Lecomte; Anne Masset; Silvia Blacher; Ludovic Maertens; André Gothot; Marie Delgaudine; Françoise Bruyère; Oriane Carnet; Jenny Paupert; Martin Illemann; Jean-Michel Foidart; Ida K Lund; Gunilla Høyer-Hansen; Agnes Noel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neoplasia (New York, N.Y.) Volume: 14 ISSN: 1476-5586 ISO Abbreviation: Neoplasia Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100886622 Medline TA: Neoplasia Country: Canada |
Other Details:
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Languages: eng Pagination: 943-51 Citation Subset: IM |
Affiliation:
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Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liège, Liège, Belgium. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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