Document Detail

Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro.
MedLine Citation:
PMID:  21542803     Owner:  NLM     Status:  MEDLINE    
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MRC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by RT-PCR (reverse transcription-PCR) analysis after day 15. Immunostaining analysis revealed the present of scattered SPC-positive cells with a differentiation efficiency of 2.43-4.21%. Our study further showed that the SPC gene expression level in differentiated cells was related to the ratio of BMSCs to MRC-5 cells and the components of modified SAGM.
Nan Ma; Hui Gai; Ju Mei; Fang-Bao Ding; Chun-Rong Bao; David M Nguyen; Hong Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-10     Completed Date:  2012-06-06     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1261-6     Citation Subset:  IM    
Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Peoples Republic of China.
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MeSH Terms
Bone Marrow Cells / cytology*,  metabolism
Cell Differentiation*
Coculture Techniques
Mesenchymal Stem Cells / cytology*,  metabolism
Pneumocytes / cytology*,  metabolism
Pulmonary Alveoli / cytology,  metabolism
Pulmonary Surfactant-Associated Protein C / genetics*,  metabolism
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein C; 0/SFTPC protein, human

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