| Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro. | |
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MedLine Citation:
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PMID: 21542803 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MRC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by RT-PCR (reverse transcription-PCR) analysis after day 15. Immunostaining analysis revealed the present of scattered SPC-positive cells with a differentiation efficiency of 2.43-4.21%. Our study further showed that the SPC gene expression level in differentiated cells was related to the ratio of BMSCs to MRC-5 cells and the components of modified SAGM. |
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Authors:
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Nan Ma; Hui Gai; Ju Mei; Fang-Bao Ding; Chun-Rong Bao; David M Nguyen; Hong Zhong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-10 Completed Date: 2012-06-06 Revised Date: 2012-08-02 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 1261-6 Citation Subset: IM |
Affiliation:
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Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Peoples Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bone Marrow Cells
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cytology*,
metabolism Cell Differentiation* Coculture Techniques Humans Mesenchymal Stem Cells / cytology*, metabolism Pneumocytes / cytology*, metabolism Pulmonary Alveoli / cytology, metabolism Pulmonary Surfactant-Associated Protein C / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Pulmonary Surfactant-Associated Protein C; 0/SFTPC protein, human |
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