Document Detail


Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro.
MedLine Citation:
PMID:  21542803     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MRC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by RT-PCR (reverse transcription-PCR) analysis after day 15. Immunostaining analysis revealed the present of scattered SPC-positive cells with a differentiation efficiency of 2.43-4.21%. Our study further showed that the SPC gene expression level in differentiated cells was related to the ratio of BMSCs to MRC-5 cells and the components of modified SAGM.
Authors:
Nan Ma; Hui Gai; Ju Mei; Fang-Bao Ding; Chun-Rong Bao; David M Nguyen; Hong Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-10     Completed Date:  2012-06-06     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1261-6     Citation Subset:  IM    
Affiliation:
Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Cells / cytology*,  metabolism
Cell Differentiation*
Coculture Techniques
Humans
Mesenchymal Stem Cells / cytology*,  metabolism
Pneumocytes / cytology*,  metabolism
Pulmonary Alveoli / cytology,  metabolism
Pulmonary Surfactant-Associated Protein C / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein C; 0/SFTPC protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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