Document Detail

Bonding erythrocytes to plastic substrates by glow-discharge activation.
MedLine Citation:
PMID:  117847     Owner:  NLM     Status:  MEDLINE    
A procedure was developed for attaching erythrocytes to various plastics. Plastic disks were exposed to ammonia glow discharge (AGD) and found thereafter to form weak bonds with red cells settling from a dilute saline suspension poured over the disks. When the disks were used to produce a rotating shear flow, complete cell detachment was observed for stresses exceeding 34 dynes/cm2. Far stronger bonding was achieved by treating AGD-bonded cells with glutaraldehyde, and subsequent shearing failed to detach any cells (maximum stress 58 dynes/cm2). The proposed chemical mechanism involves attachment of -NH2 groups on the plastic during AGD, and formation also of carbonyl oxygen later, to provide sites for hydrogen bonding with the cell membrane. Subsequent glutaraldehyde exposure produces crosslinking between disk and cell, as well as fixing the cell in the normal way. Thus, the initial step succeeds in attaching erythrocytes in a deformable condition but rather weakly, while the second step produces rigid cells which are also bonded more strongly.
R W Benedict; M C Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomaterials, medical devices, and artificial organs     Volume:  7     ISSN:  0090-5488     ISO Abbreviation:  Biomater Med Devices Artif Organs     Publication Date:  1979  
Date Detail:
Created Date:  1980-03-24     Completed Date:  1980-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0356630     Medline TA:  Biomater Med Devices Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  477-93     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion
Cross-Linking Reagents
Erythrocytes / physiology*
Glutaral / pharmacology
Polyvinyl Chloride
Stress, Mechanical
Surface Properties
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Plastics; 0/Polyethylenes; 0/Polypropylenes; 111-30-8/Glutaral; 7664-41-7/Ammonia; 9002-86-2/Polyvinyl Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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