Document Detail

Bombesin and nutrients stimulate release of CCK through distinct pathways in the rat.
MedLine Citation:
PMID:  2735417     Owner:  NLM     Status:  MEDLINE    
An isolated, vascularly perfused duodenojejunum model was developed in the rat to investigate the mechanisms involved in the release of cholecystokinin (CCK) by vascular bombesin (BBS) and luminal nutrients (LN). Immunoreactive peptides released into the portal vein effluent were measured with three antisera that were relatively CCK specific, gastrin specific, and cross-reactive to both CCK and gastrin, respectively. Bombesin (10(-7) M) provoked a biphasic release of CCK, consisting of a transient rise (approximately 700% above basal) followed by a sustained response (approximately 250% above basal). Gastrin accounted for less than 25% of the total immunoreactivity as measured with the nonspecific antiserum. The two phases of the CCK response were related to the dose of BBS in the range 10(-10)-10(-6) M, with a half-maximal effect at 10(-8) M BBS. Tetrodotoxin (TTX, 10(-6) M) reduced the second phase only (by 80%), whereas hexamethonium (10(-4) M) and atropine (10(-5) M) left the two phases unaltered. LN induced a prompt and well-sustained release of CCK (approximately 250% above basal) that was not affected by arterial TTX or atropine. These results demonstrate that BBS stimulated the release of CCK through both a direct pathway and an indirect, noncholinergic mechanism, while the effect of LN did not appear to involve intramural nerves.
J C Cuber; F Vilas; N Charles; C Bernard; J A Chayvialle
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-07-18     Completed Date:  1989-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G989-96     Citation Subset:  IM    
Institut National de la Santé et de la Recherche Médicale U45, Hôpital E. Herriot, Lyon, France.
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MeSH Terms
Atropine / pharmacology
Bombesin / administration & dosage,  pharmacology*
Cholecystokinin / secretion*
Culture Media
Duodenum / drug effects,  secretion*
Gastrins / secretion
Infusions, Intra-Arterial
Jejunum / drug effects,  secretion*
Rats, Inbred Strains
Tetrodotoxin / pharmacology
Reg. No./Substance:
0/Culture Media; 0/Gastrins; 31362-50-2/Bombesin; 4368-28-9/Tetrodotoxin; 51-55-8/Atropine; 9011-97-6/Cholecystokinin

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