Document Detail


Boehringer Mannheim modular test concepts in HIV and hepatitis immunoassays.
MedLine Citation:
PMID:  7694814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anti-HIV-antibody and hepatitis C virus (HCV)-antibody screening tests have to be able to detect a variety of virus antibodies. On the other hand, HIV-antigen specific antibody tests that detect only one kind of antibody are needed for prognosis of disease or for distinguishing infection by different virus subtypes. Usually in an enzyme-linked immunosorbent assay for each individual test an individual solid phase has to be created. For our Boehringer Mannheim Enzymun-Test Diagnostics Assay we used a single universal biotin-binding solid phase in all tests and biotin-labeled specific antigens for the individual tests. The modular system for the antibody tests is a convenient tool for the development of a broad test menu for different viruses. We show that the modular system is suited for screening tests for HIV1, HIV2, or HCV antibodies, as as well as for virus typing or for detection of HIV/p24-specific antibodies in a quantitative assay with high precision.
Authors:
U Wienhues; E Faatz; W Melchior; H Bayer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical biochemistry     Volume:  26     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1994-01-04     Completed Date:  1994-01-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  295-9     Citation Subset:  IM; X    
Affiliation:
Boehringer Mannheim GmbH, Testentwicklung Werk Tutzing, Germany.
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Enzyme-Linked Immunosorbent Assay / methods*
HIV Antibodies / blood*
HIV Core Protein p24 / blood
HIV Infections / blood,  immunology*
HIV-1*
HIV-2*
Hepatitis Antibodies / blood*
Hepatitis C Antibodies
Humans
Lupus Erythematosus, Systemic / blood,  immunology*
Sensitivity and Specificity
Virology / methods*
Chemical
Reg. No./Substance:
0/HIV Antibodies; 0/HIV Core Protein p24; 0/Hepatitis Antibodies; 0/Hepatitis C Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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