| Body temperature and heat production in suckling rat endotoxaemia: beneficial effects of glutamine. | |
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MedLine Citation:
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PMID: 12592615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Sepsis is an important cause of neonatal mortality. The aim of the study was to investigate the metabolism of endotoxic neonatal rats and the potential beneficial effect of glutamine. METHODS: Suckling rats received intraperitoneal saline (control; C), endotoxin (300 microg/g LPS; E), saline+glutamine (2 mmol/g; CG), endotoxin+glutamine (EG), saline+leucine (2 mmol/g; CL) or endotoxin+leucine (EL). Sepsis score (0-8) and rectal temperature were monitored. Hypothermia was defined as rectal temperature less than 32 degrees C. Oxygen consumption (VO2, mL/kg/h), a determinant of heat production, was measured by indirect calorimetry. Data (mean +/- SEM) were compared by analysis of variance (ANOVA), paired t test or Fisher's Exact test. RESULTS: Endotoxic (E) rats had significantly lower VO2 than C rats from 90 minutes postinjection to the end of the experiment, 210 minutes (VO2 from 150 to 210 minutes: C 671 +/- 45; E 429 +/- 36, P <.0004; n = 8; paired t test). VO2 of CL or CG rats was elevated between 90 and 210 minutes compared with control, but significantly (P <.01) only in the L group (C 706 +/- 31; CG 871 +/- 63; CL 984 +/- 31; n = 7-9, ANOVA). VO2 was significantly higher (P <.05) in EG rats than E rats (E 460 +/- 29; EG 654 +/- 68; n = 9-10). In the EL group, VO2 was raised but was not significantly different from E (E 460 +/- 29; EL 637 +/- 52; n = 8-10). EG rats were significantly less hypothermic between 90 and 210 minutes (58 of 132 measurements) compared with E (95 of 147; P =.0007, Fisher's Exact test), whereas the EL group were similarly hypothermic (74 of 120) to E (P =.7). Sepsis score was significantly lower in the EG group than both E and EL groups (E 4.9 +/- 0.3; EG 3.6 +/- 0.3; EL 5.0 +/- 0.3; n = 40; P <.01; ANOVA). CONCLUSIONS: Neonatal endotoxaemia lowers VO2, heat production, and body temperature. Glutamine and leucine both cause nutrient-induced thermogenesis in control animals and restore VO2 of endotoxic animals. Glutamine additionally increases rectal temperature, reduces incidence of hypothermia, and improves clinical signs of endotoxic rats. This suggests that glutamine may be beneficial for nutrition in neonatal sepsis. |
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Authors:
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Robin G Garrett-Cox; Agostino Pierro; Lewis Spitz; Simon Eaton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 38 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-02-19 Completed Date: 2003-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 37-44; discussion 37-44 Citation Subset: IM |
Copyright Information:
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Copyright 2003, Elsevier Science (USA). All rights reserved. |
Affiliation:
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Department of Paediatric Surgery, Institute of Child Health, London, England. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Suckling Basal Metabolism / drug effects, physiology Body Temperature / drug effects*, physiology Carbon Dioxide / metabolism Endotoxemia / drug therapy*, metabolism, physiopathology Energy Intake / drug effects, physiology Energy Metabolism / drug effects, physiology Glutamine / pharmacology, therapeutic use* Leucine / pharmacology, therapeutic use Oxygen Consumption / drug effects, physiology Rats Rats, Wistar Rectum / drug effects, physiology Thermogenesis / drug effects*, physiology |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide; 56-85-9/Glutamine; 61-90-5/Leucine |
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