Document Detail


Body mass index-independent inflammation in omental adipose tissue associated with insulin resistance in morbid obesity.
MedLine Citation:
PMID:  20678967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Obesity is a strong risk factor for resistance to insulin-mediated glucose disposal, a precursor of type 2 diabetes and other disorders. However, not all obese individuals are insulin resistant. We sought to identify the molecular pathways that might cause obesity-associated insulin resistance in humans by studying the morbidly obese who were insulin sensitive versus insulin resistant, thereby eliminating obesity as a variable.
METHODS: Combining gene expression profiling with computational approaches, we determined the global gene expression signatures of omental and subcutaneous adipose tissue samples obtained from similarly obese patients undergoing gastric bypass surgery.
RESULTS: Gene sets related to chemokine activity and chemokine receptor binding were identified as most highly expressed in the omental tissue from insulin-resistant compared with insulin-sensitive subjects, independent of the body mass index. These upregulated genes included chemokines (C-C motif) ligand 2, 3, 4, and 18 and interleukin-8/(CC-X motif) ligand 8 and were not differentially expressed in the subcutaneous adipose tissues between the 2 groups of subjects. Insulin resistance, but not the body mass index, was associated with increased macrophage infiltration in the omental adipose tissue, as was adipocyte size, in these morbidly obese subjects.
CONCLUSION: Our findings have demonstrated that inflammation of the omental adipose tissue is strongly associated with insulin resistance in human obesity even in subjects with similar body mass index values.
Authors:
Olga T Hardy; Richard A Perugini; Sarah M Nicoloro; Karen Gallagher-Dorval; Vishwajeet Puri; Juerg Straubhaar; Michael P Czech
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-01
Journal Detail:
Title:  Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery     Volume:  7     ISSN:  1878-7533     ISO Abbreviation:  Surg Obes Relat Dis     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2011-01-24     Completed Date:  2011-05-24     Revised Date:  2012-04-13    
Medline Journal Info:
Nlm Unique ID:  101233161     Medline TA:  Surg Obes Relat Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Society for Metabolic and Bariatric Surgery. Published by Elsevier Inc. All rights reserved.
Affiliation:
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / metabolism,  pathology
Adipose Tissue / metabolism*,  pathology
Adult
Body Mass Index*
Chemokines / biosynthesis,  genetics
Female
Follow-Up Studies
Gene Expression
Humans
Inflammation / genetics,  metabolism*,  pathology
Insulin Resistance*
Male
Middle Aged
Obesity, Morbid / metabolism*,  physiopathology
Omentum*
Polymerase Chain Reaction
RNA / genetics
Retrospective Studies
Grant Support
ID/Acronym/Agency:
DK30898/DK/NIDDK NIH HHS; DK32520/DK/NIDDK NIH HHS; KL2 RR031981-02/RR/NCRR NIH HHS; P30 DK032520-26/DK/NIDDK NIH HHS; R01 DK030898-28/DK/NIDDK NIH HHS; UL1 RR031982-02/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Chemokines; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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