Document Detail

Body composition and serum levels of adiponectin, vascular endothelial growth factor, and interleukin-6 in patients with rheumatoid arthritis.
Jump to Full Text
MedLine Citation:
PMID:  22911528     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate differences in body composition and body mass index (BMI) in patients with rheumatoid arthritis (RA) and their correlations with serum production of adiponectin, interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF).
METHODS: The study included 83 patients (age 53±5 years) with RA treated with methotrexate. We determined their BMI, fat mass, and fat-free mass using bioimpedance analysis, and serum concentrations of adiponectin, VEGF, and IL-6 using immunoassay analysis.
RESULTS: Normal BMI was found in 39 (47%), overweight and obesity in 26 (31%), and underweight in 18 (22%) patients. Concentration of adiponectin was lower in overweight/obese patients than in patients with normal BMI (2.1 [0.8-3.9] μg/mL vs 8.9 (7.2-11.3) μg/mL). In underweight patients, it was moderately increased (12.7 [9.3-14.8] μg/mL) and the correlation between the concentrations of adiponectin and IL-6 was positive (r=0.4; P=0.01). Concentrations of VEGF and IL-6 were increased in all groups with RA. The overweight/obese group showed a negative correlation between the concentrations of adiponectin and VEGF (r=- 0.34; P=0.04), a positive correlation between VEGF concentration and fat mass (r=0.39; P=0.02), and a negative correlation between adiponectin concentration and fat mass (r=- 0.23; P=0.02).
CONCLUSION: Inflammatory and angiogenesis activation was found in RA patients with all types of body composition, but only in those with obesity and overweight there was a direct antagonism between adiponectin and VEGF. Further research is needed to identify possible regimens of metabolic correction in different variations of body composition.
Sergey P Oranskiy; Ludmila N Yeliseyeva; Anna V Tsanaeva; Nadezhda V Zaytseva
Related Documents :
21959878 - Evaluation of diagnostic accuracy of portable echocardiography in newborns.
10210478 - High-resolution computed tomography in pediatric patients with postinfectious bronchiol...
21756568 - Description of sensory preservation in children and adolescents with incomplete spinal ...
22911528 - Body composition and serum levels of adiponectin, vascular endothelial growth factor, a...
15721058 - Brain processing of visual sexual stimuli in treated and untreated hypogonadal patients.
24853318 - Capsule endoscopic findings correlate with fecal calprotectin and c-reactive protein in...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Croatian medical journal     Volume:  53     ISSN:  1332-8166     ISO Abbreviation:  Croat. Med. J.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-22     Completed Date:  2013-03-04     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9424324     Medline TA:  Croat Med J     Country:  Croatia    
Other Details:
Languages:  eng     Pagination:  350-6     Citation Subset:  IM    
Kuban State Medical University, 4 Sedina Street, Krasnodar 350063, Russian Federation.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adiponectin / blood*
Arthritis, Rheumatoid / blood*
Body Composition
Body Mass Index*
Interleukin-6 / blood*
Middle Aged
Vascular Endothelial Growth Factor A / blood*
Reg. No./Substance:
0/Adiponectin; 0/Interleukin-6; 0/Vascular Endothelial Growth Factor A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Croat Med J
Journal ID (iso-abbrev): Croat. Med. J
Journal ID (publisher-id): CMJ
ISSN: 0353-9504
ISSN: 1332-8166
Publisher: Croatian Medical Schools
Article Information
Download PDF
Copyright © 2012 by the Croatian Medical Journal. All rights reserved.
Received Day: 25 Month: 12 Year: 2011
Accepted Day: 21 Month: 6 Year: 2012
Print publication date: Month: 8 Year: 2012
Volume: 53 Issue: 4
First Page: 350 Last Page: 356
ID: 3429942
PubMed Id: 22911528
Publisher Id: CroatMedJ_53_0350
DOI: 10.3325/cmj.2012.53.350

Body composition and serum levels of adiponectin, vascular endothelial growth factor, and interleukin-6 in patients with rheumatoid arthritis Alternate Title:Oransky et al:Body composition and serum levels of adiponectin, VEGF, and IL-6
Sergey P. Oranskiy
Ludmila N. Yeliseyeva
Anna V. Tsanaeva
Nadezhda V. Zaytseva
Kuban State Medical University, Krasnodar, Russian Federation
Correspondence: Correspondence to:
Nadezhda V. Zaytseva
Kuban State Medical University
4 Sedina Street
Krasnodar 350063
Russian Federation

Rheumatoid arthritis (RА) is one of the most frequent autoimmune diseases, with up to 1.3% prevalence in the world, which is also associated with a high risk of disability (1). The leading cause of death in RA patients are cardiovascular diseases (2,3), which can be related to changes of nutritional and metabolic status and correlate with body mass index (BMI). However, little is known about the impact of different metabolic factors on body composition in RA patients (4,5).

According to the World Health Organization (WHO) criteria, there are three types of metabolic phenotypes as determined by BMI – normal BMI, underweight, and obesity (6). WHO criteria do not embrace the term “cachexia,” but this is one of the most discussed problems in patients with RA. One of the latest definitions was: “Cachexia is a complex metabolic syndrome associated with underlying illness and characterized by loss of muscle with or without loss of fat mass” (7), but there is no generally accepted definition of cachexia in RA patients. Cachexia in RA is divided into two types – the “classic” low-BMI type (with both low muscle mass and low fat mass) and the more frequent type termed “rheumatoid cachexia,” associated with normal or increased BMI (characterized by low muscle mass but increased fat mass) (8). Many RA patients with or without cachexia have protein energy insufficiency and reduction of muscular and/or fat mass, which is connected with a hypercatabolism state and proinflammatory activation (9). The problem of metabolic syndrome and obesity in patients with RА has recently attracted more research attention (10) and it seems that the frequency of these conditions is quite high (11).

Metabolic changes can be associated with the effects of adipokines. One of the key adipokines is adiponectin, a cytokine-like protein hormone and one of the main regulators of carbohydrate and fat exchange, produced exclusively in the fat tissue. Adiponectin has structural amino acid sequences homologous with collagens, some complement factors, and tumor necrosis factor-α (12). Decreasing adiponectin production leads to insulin resistance, because of its ability to inhibit gluconeogenesis and to stimulate oxidation of free fat acids in skeletal muscles and the liver (13). So, the adiponectin level decreases in patients with obesity, metabolic syndrome, type II diabetes mellitus, and cardiovascular diseases (14). In autoimmune diseases, adiponectin acts also as a cytokine regulator with functional dualism and the ability to influence proinflammatory and anti-inflammatory mediators (cytokines, growth factors, etc.) (15,16).

Increased production of serum adiponectin and correlation of adiponectin with proinflammatory cytokines was found in RА patients with normal body weight (17,18), but only one study investigated the production of serum adiponectin in RA patients with obesity (19). Also, most studies identified increased serum concentrations of IL-6 and VEGF in RA patients with normal body weight (20-22), without studying the patients with reduced or increased BMI. Choi et al (18) found a direct effect of adiponectin on the stimulation of VEGF in the synovial fluid of RA patients. Serum concentrations of adiponectin, VEGF, and IL-6 have not yet been investigated in RA patients with different BMI and body composition variations. The aim of this study was to investigate differences in body composition and BMI in patients with RA and their correlations with serum production of adiponectin, IL-6, and VEGF.

Materials and methods

The study included 83 patients admitted to the Rheumatology Department of Kuban State Medical University and Krasnodar Regional Hospital (15 men, 68 women) with seropositive RА. They were divided into 3 groups: patients with normal BMI (n = 39), patients with obesity and overweight (n = 26), and patients with underweight (n = 18). Patients with obesity and overweight were fused in one group due to their similar effect on serum adiponectin and proinflammatory cytokine status in general population (23). The control group (20 persons – 4 men and 16 women) was formed from healthy blood donors and randomized with RA groups using stratified randomization by age and sex. RA groups and the control group did not differ according to demographic characteristics. RA groups did not differ according to clinical characteristics and medications (Table 1). We excluded the patients with concomitant infectious, oncological diseases, purulent conditions of any localization, and those with renal, hepatic, or cardiac insufficiency. To eliminate the influence of nutritional factors on BMI and body composition we used the Mini Nutritional Assessment (24). There were no differences between the groups of RA patients according to all indicators of this questionnaire. The majority of RA patients received methotrexate as a disease-modifying drug, without differences in dosages and duration of therapy between RA groups. Also, to avoid the influence of symptomatic therapy on body composition, we retrospectively quantified the cumulative dosage of nonsteroidal anti-inflammatory drugs (NSAID) and corticosteroids. Cumulative dosage was calculated as: daily dosage × number of days of dosing. We did not register any differences in duration and cumulative dosage between RA groups. Body composition indicators – BMI, fat mass, and fat-free mass – were determined using bioimpedance analysis (TANITA BC-418 analyzer, Tanita Corporation, Tokyo, Japan). The study was approved by the Ethics Committee of the Human Studies of Kuban State Medical University (Krasnodar, Russia) and was conducted according to principles of the Helsinki Declaration of the World Medical Association (2008 revision). Informed consent was received from all patients and the control group.

Patients were admitted to our research unit between September 12, 2009 and September 12, 2011, and a detailed history and physical examination was taken. We assessed the disease activity with the Disease Activity Score (DAS28), including tender and swollen joint count and general health status (25). After overnight fasting, hemogram and leukocyte count, erythrocyte sedimentation rate (ESR), elemental serum biochemistry (electrolytes, urea, creatinine, albumin, standard liver function tests, transaminases, bilirubin, albumin), rheumatoid factor (RF), and C-reactive protein (CRP) were determined. Blood samples were collected in sterile Vacutainer tubes, centrifuged at 3500 rpm for 15 minutes at 4°C, and stored at -80°C in pyrogen-free polyethylene tubes. Body mass index (BMI) was calculated according to the formula: [body weight/height2] (kg/m2). Due to the lack of generally accepted criteria for rheumatoid cachexia, we divided the patients with RA according to the WHO criteria (6). In accordance with the WHO standards, patients with BMI values lower than 18.5 kg/m2 were classified as underweight, with values between 18.5 and 24.9 as normal, with values between 25-29.9 kg/m2 as overweight, and with values greater than 30 as obese.

We measured the serum concentration of adiponectin, VEGF, and IL-6 using the BioVendor (Modrice, Czech Republic), Invitrogen (Camarillo, CA, USA), and Protein Contour Ltd (Saint-Petersburg, Russian Federation) kits and using immune-enzyme analysis (analyzer Statfax 2100, Awareness Technology Inc., Palm City, FL, USA). All data were processed using Statistica 6.0. (Statsoft, Tulsa, OK, USA). Considering the asymmetrical shape of distribution (Kolmogorov-Smirnov test), data were presented as medians and ranges. Differences between unpaired samples were measured with the nonparametric Mann-Whitney test. We also performed a correlation analysis between all parameters studied in patients with RA. Correlations were determined with the Spearman rank order correlation test.


Patients in all groups had medium disease activity according to general RA markers – DAS28, ESR, CRP, and most of them were treated with methotrexate as disease-modifying drug. There were no differences in duration, dosage of corticosteroids, or NSAIDs treatment (Table 1).

Thirty nine (47%) patients had normal BMI, 26 (31%) were obese and overweight, and 18 (22%) were underweight (Table 1). There were no differences in fat mass and fat-free mass between patients with normal BMI and the control group. Only overweight and obese patients had high fat mass and low fat-free mass. This means that this group presented with rheumatoid cachexia. Underweight group had lower fat mass and lower fat-free mass (classic low BMI type of rheumatoid cachexia) than the control group and the group with normal BMI (Table 2).

The concentration of adiponectin was higher in the group with normal BMI than in overweight and obese patients (8.9 [7.2-11.3] vs 2.1 [0.8-3.9] μg/mL) and significantly lower than in underweight patients (12.7 [9.3-14.8] μg/mL). The concentration of IL-6 was higher in all groups with RА than in controls; however, in the group with obesity it was higher than in the group with normal BMI, and in the group with normal BMI it was higher than in underweight patients (Table 3). Underweight patients had a positive correlation between adiponectin and IL-6 serum concentrations (r = 0.4; P = 0.01).

The concentration of VEGF was higher in all RA groups than in the control group. However, the group with obesity and overweight had higher VEGF values than the normal BMI and control group (Table 3). The group with obesity and overweight had a negative correlation between adiponectin and VEGF concentrations (r = -0.34; P = 0.04), a positive correlation between VEGF concentration and fat mass (r = 0.39; P = 0.02), and a negative correlation between adiponectin concentration and fat mass (r = - 0.23; P = 0.02).


In our study, patients with overweight and obesity had rheumatoid cachexia, with reduced fat-free mass and increased fat mass. The underweight group had classic low BMI type of rheumatoid cachexia. This is in accordance with the findings of Elkan et al (9), except that in our study patients with normal BMI had no rheumatoid cachexia.

Adiponectin production in adults has been found to inversely correlate with body fat percentage (26), but the data in patients with RA are limited. Adiponectin is known to play an important role in the pathogenesis of RA, although its action as an anti-inflammatory or pro-inflammatory mediator is controversial (14-16). In general population, low adiponectin levels have been associated with obesity, type 2 diabetes mellitus, atherosclerosis, and metabolic syndrome, and its role in these conditions is clearly anti-inflammatory (27-29). On the other hand, some authors have reported high adiponectin levels correlating with disease activity in general population of RA patients (30). Only one study (19) demonstrated hypoadiponectinemia in patients with RA and obesity, which is similar to our data. In our study, serum concentration of adiponectin increased in RA patients with normal BMI and underweight patients and decreased in obesity/overweight patients.

Another important aspect of adiponectin production is the potential impact of different local (joint) and systemic (serum) effects. At the joint level, adiponectin contributed to synovitis and joint destruction in patients with RA (without specifying body composition type) by stimulating VEGF and matrix metalloproteinases expression in fibroblast-like synoviocytes (18). We found a negative correlation between adiponectin and VEGF serum concentration in obese/overweight patients and a positive correlation between adiponectin and IL-6 serum concentration in underweight patients. Until recently, data about the link between VEGF, other angiogenic factors, and cardiovascular risk were contradictory. A recent study has demonstrated an association between VEGF gene polymorphisms and RA (31). Another study has found no significant association between the VEGFA rs2010963 and s1570360 polymorphisms and clinically evident cardiovascular disease (32). In yet another study, levels of VEGF have been negatively associated with 10-year cardiovascular disease and stroke risk (33). There are no unanimous data on the concentration of IL-6 in different types of body composition in the general population of RA patients. In our study, serum concentration of VEGF and IL-6 was increased in all types of body composition. We also found a lower level of IL-6 in underweight patients than in normal BMI and overweight patients. IL-6 level in RA patients may be related to genetic polymorphism as well. In patients with RA associated with obesity or smoking, IL-6 levels were significantly increased in C-allele carriers, but no significant interactions were recorded between adiposity and IL6-174G/C genotypes (34).

We registered all types of body composition and BMI variations (normal BMI, overweight/obesity, and underweight) in RA patients. Patients with overweight/obesity and underweight had rheumatoid cachexia. All BMI variations were associated with IL-6 and VEGF activation but only in the group with overweight and obesity we found correlations between fat mass and serum concentrations of adiponectin and VEGF, which may point to a more active stimulation of angiogenesis and vasculature and inhibition of a key adipocytokine. Further research is required to identify possible regimens of metabolic correction in different variations of body composition.

Funding The study was financially supported by the Research Department of Kuban State Medical University (Krasnodar, Russian Federation), grant No. 5 (2010).

Ethical approval received from the Ethics Committee on Human studies of Kuban State Medical University (Krasnodar, Russia).

Declaration of authorship SPO was a principal investigator and participated in study design, acquisition of data, analysis and interpretation, manuscript preparation, and statistical analysis. LNY was a principal investigator and participated in study design, acquisition of data, analysis and interpretation, manuscript preparation, and statistical analysis. AVT was an investigator and participated in manuscript preparation and statistical analysis. NVZ translated and edited this manuscript.

Competing interests All authors have completed the Unified Competing Interest form at (available on request from the corresponding author) and declare: no support from any organization for the submitted work; no financial relationships with any organizations that might have an interest in the submitted work in the previous 3 years; no other relationships or activities that could appear to have influenced the submitted work.

1. MacGregor AJ, Silman AJ. Classification and epidemiology. In: Hochberg MC, Silman AJ, Smolen JS, Weinblatt ME, Weisman MH, eds. Rheumatology. 4th ed. Philadelphia, PA: Mosby Elsevier; 2008.
2. Avina-Zubieta JA,Choi HK,Sadatsafavi M,Etminan M,Esdaile JM,Lacaille D. Risk of cardiovascular mortality in patients with rheumatoid arthritis: a meta-analysis of observational studies.Arthritis RheumYear: 2008591690710.1002/art.2409219035419
3. Naranjo A,Sokka T,Descalzo MA,Calvo-Alen J,Horslev-Petersen K,Luukkainen RK,et al. Cardiovascular disease in patients with rheumatoid arthritis: results from the QUEST-RA study.Arthritis Res TherYear: 200810R3010.1186/ar238318325087
4. Hu G,Qiao Q,Tuomilehto J,Balkau B,Borch-Johnsen K,Pyorala K. Prevalence of The metabolic syndrome and its relation to all cause and cardiovascular mortality in nondiabetic European men and women.Arch Intern MedYear: 200416410667610.1001/archinte.164.10.106615159263
5. Gomez-Vaquero C,Nolla JM,Fiter J,Ramon JM,Concustell R,Valverde J,et al. Nutritional status in patients with rheumatoid arthritis.Joint Bone SpineYear: 200168403910.1016/S1297-319X(01)00296-211707006
6. World Health Organization Report. Obesity: preventing and managing the global epidemic. WHO Obesity Technical Report Series 894. Geneva (Switzerland): World Health Organization; 2000.
7. Evans WJ,Morley JE,Argiles J,Bales C,Baracos V,Guttridge D,et al. Cachexia: a new definition.Clin NutrYear: 200827793910.1016/j.clnu.2008.06.01318718696
8. Summers GD,Metsios GS,Stavropoulos-Kalinoglou A,Kitas GD. Rheumatoid cachexia and cardiovascular disease.Nat Rev Rheumatol.Year: 201064455110.1038/nrrheum.2010.10520647995
9. Elkan AC,Engvall IL,Hafstrom I. Rheumatoid cachexia, central obesity and malnutrition in patients with low-active rheumatoid arthritis: feasibility of anthropometry, Mini Nutritional Assessment and body composition techniques.Eur J NutrYear: 2009483152210.1007/s00394-009-0017-y19333642
10. Pereira RM,de Carvalho JF,Bonfa E. Metabolic syndrome in rheumatological diseases.Autoimmun RevYear: 20098415910.1016/j.autrev.2009.01.00119168158
11. Karvounaris SA,Sidiropoulos PI,Papadakis JA,Spanakis EK,Bertsias GK,Kritikos HD,et al. Metabolic syndrome is common among middle-to-older-age Mediterranean patients with rheumatoid arthritis and correlates with disease activity: a retrospective, cross-sectional, controlled study.Ann Rheum DisYear: 200766283310.1136/ard.2006.05348816793841
12. Ceddia RB,Somwar R,Maida A,Fang X,Bikopoulo G,Sweeney G. Globular adiponectin increases GLUT4 translocation and glucose uptake but reduces glycogen synthesis in rat skeletal muscle cells.DiabetologiaYear: 200548132910.1007/s00125-004-1609-y15619075
13. Katagiri H,Yamada T,Oka Y. Adiposity and cardiovascular disorders: disturbance of the regulatory system consisting of humoral and neuronal signals.Circ ResYear: 2007101273910.1161/CIRCRESAHA.107.15162117615379
14. Bruun JM,Lihn AS,Verdich C,Pedersen SB,Toubro S,Astrup A,et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans.Am J Physiol Endocrinol MetabYear: 2003285E5273312736161
15. Ouchi N,Parker JL,Lugus JJ,Walsh K. Adipokines in inflammation and metabolic disease.Nat Rev ImmunolYear: 201111859710.1038/nri292121252989
16. Otero M,Lago R,Gomez R,Lago F,Dieguez C,Gomez-Reino JJ,et al. Changes in plasma levels of fat-derived hormones adiponectin, leptin, resistin and visfatin in patients with rheumatoid arthritis.Ann Rheum DisYear: 200665119820110.1136/ard.2005.04654016414972
17. Ozgen M,Koca SS,Dagli N,Balin M,Ustundag B,Isik A. Serum adiponectin and vaspin levels in rheumatoid arthritis.Arch Med ResYear: 2010414576310.1016/j.arcmed.2010.08.01221044750
18. Choi HM,Lee YA,Lee SH,Hong SJ,Hahm DH,Choi SY,et al. Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators.Arthritis Res TherYear: 200911R1617110.1186/ar284419883500
19. Fagerer N,Kullich W. Adipocytokines in rheumatoid arthritis and obesity.Wien Med WochenschrYear: 2010160391810.1007/s10354-010-0808-z20812050
20. Schroeder M,Viezens L,Fuhrhop I,Ruther W,Schaefer C,Schwarzloh B,et al. Angiogenic growth factors in rheumatoid arthritisRheumatol IntYear: 2011Nov 9. [Epub ahead of print]10.1007/s00296-011-2210-622068355
21. Hashizume M,Mihara M.. The roles of interleukin-6 in the pathogenesis of rheumatoid arthritisArthritisYear: 2011201176562410.1155/2011/76562422046525
22. Rho YH,Chung CP,Oeser A,Solus J,Asanuma Y,Sokka T,et al. Inflammatory mediators and premature coronary atherosclerosis in rheumatoid arthritis.Arthritis RheumYear: 2009611580510.1002/art.2500919877084
23. Loria Kohen V,Gómez Candela C,Fernández Fernández C,Zurita Rosa L,Palma Milla S,Urbieta M,et al. Hormonal and inflammatory biomarkers in a group of overweight and obese women.Nutr HospYear: 201126884922470038
24. Kaiser MJ,Bauer JM,Ramsch C,Uter W,Guigoz Y,Cederholm T,et al. Validation of the Mini Nutritional Assesment short-form (MNA-SF): a practical tool for identification of nutritional status.J Nutr Health AgingYear: 200913782810.1007/s12603-009-0214-719812868
25. Wells G,Becker JC,Teng J,Dougados M,Schiff M,Smolen J,et al. Validation of the 28-joint Disease Activity Score (DAS28) and European League Against Rheumatism response criteria based on C-reactive protein against disease progression in patients with rheumatoid arthritis, and comparison with the DAS28 based on erythrocyte sedimentation rate.Ann Rheum DisYear: 2009689546010.1136/ard.2007.08445918490431
26. Ukkola O,Santaniemi M. Adiponectin: a link between excess adiposity and associated comorbidities?J Mol MedYear: 20028069670210.1007/s00109-002-0378-712436346
27. Kopp HP,Krzyzanowska K,Mohlig M,Spranger J,Pfeiffer AF,Schernthaner G. Effects of marked weight loss on plasma levels of adiponectin, markers of chronic subclinical inflammation and insulin resistance in morbidly obese women.Int J Obes (Lond)Year: 2005297667110.1038/sj.ijo.080298315917853
28. Weyer C,Funahashi T,Tanaka S,Hotta K,Matsuzawa Y,Pratley RE,et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia.J Clin Endocrinol MetabYear: 2001861930510.1210/jc.86.5.193011344187
29. Shibata R,Sato K,Pimentel DR,Takemura Y,Kihara S,Ohashi K,et al. Adiponectin protects against myocardial ischemia-reperfusion injury through AMPK- and COX-2-dependent mechanisms.Nat MedYear: 200511109610310.1038/nm129516155579
30. Ebina K,Fukuhara A,Ando W,Hirao M,Koga T,Oshima K,et al. Serum adiponectin concentrations correlate with severity of rheumatoid arthritis evaluated by extent of joint destruction.Clin RheumatolYear: 2009284455110.1007/s10067-008-1074-y19085030
31. Lv HZ,Lin T,Xia LP,Shen H,Zhu XY,Zhang JT,et al. Vascular endothelial growth factor gene polymorphisms and rheumatoid arthritis.J Investig MedYear: 201159593821245770
32. Rodriguez-Rodriguez L,Garcia-Bermudez M,Gonzalez-Juanatey C,Vazquez-Rodriguez TR,Miranda-Filloy JA,Fernandez-Gutierrez B,et al. Vascular endothelial growth factor A and cardiovascular disease in rheumatoid arthritis patients.Tissue AntigensYear: 201177291710.1111/j.1399-0039.2010.01625.x21388351
33. Bhatia GS,Sosin MD,Patel JV,Grindulis KA,Khattak FH,Davis RC,et al. Plasma indices of angiogenesis in rheumatoid disease: relationship to cardiovascular risk factors and cardiac function.Int J CardiolYear: 2010145e105810.1016/j.ijcard.2008.12.16819185362
34. Panoulas VF,Stavropoulos-Kalinoglou A,Metsios GS,Smith JP,Milionis HJ,Douglas KM,et al. Association of interleukin-6 (IL-6)-174G/C gene polymorphism with cardiovascular disease in patients with rheumatoid arthritis: the role of obesity and smoking.AtherosclerosisYear: 20092041788310.1016/j.atherosclerosis.2008.08.03618848327

[TableWrap ID: T1] Table 1 

Basic demographic and clinical characteristics of the study cohort*

Parameter Controls (n = 20) Patients with normal BMI (n = 39) Patients with obesity and overweight (n = 26) Underweight patients (n = 18)
Percent of women
Age in years (median and range)
52.0 (50.0-60.0)
53.0 (51.0-62.0)
52.0 (50.0-57.0)
55.0 (51.0-59.0)
Current smoking, n (%)
3.0 (15.0)
6.0 (15.4)
4.0 (15.4)
3.0 (16.7)
Duration of RA in years (median and range)
6.5 (4.5-9.0)
6.0 (5.0-9.0)
6.7 (5.4-10)
Rheumatoid factor positive, n (%)
ACCP positive, n (%)
34.0 (87.0)
23.0 (89.0)
15.0 (83.0)
CRP, mg/L (median and range)
20.5 (14.9-20.2)
20.0 (14.0-28.0)
22.0 (13.0-29.0)
ESR in mm/h (median and range)
8.0 (2.0-10.0)
33.5 (26.0-37.5)
20.0 (14.0-28.0)
29.0 (15.0-38.0)
DAS28 (median and range)
7.3 (6.3-7.8)
7.2 (6.9-7.7)
7.4 (6.2-8.1)
Current methotrexate, n (%)
30.0 (77.0)
21.0 (81.0)
14.0 (78.0)
Current prednisone, n (%)
24.0 (62.0)
18.0 (69.0)
13.0 (72.0)
Current NSAIDs, n (%) - 15.0 (38.0) 10.0 (42.0) 8.0 (44.0)

*Abbreviations: BMI – body mass index; RA – rheumatoid arthritis; ACCP – antibodies to cyclic citrullinated peptide; CRP – C-reactive protein; ESR – erythrocyte sedimentation rate; DAS28 – disease activity score-28; NSAID – non-steroidal anti-inflammatory drug.

[TableWrap ID: T2] Table 2 

Body composition of the study cohort patients*

Indicator (median and range) Control group (n = 20) Patients with overweight and obesity (n = 26) P Underweight patients (n = 18) P Patients with normal BMI (n = 39) P
Body mass index (BMI), kg/m2
23 (21.2-24.1)
31.8 (29.5-40.1)
0.04; 0.04
17.9 (16.5-19.2)
0.03; 0.04
25.2 (24.1-27.3)
Fat mass, kg
19.3 (17-22)
29.1 (26.5-31.2)
0.04; 0.03
17.5 (14.6-19)
0.04; 0.04
21.2 (19-25.4)
Fat mass, %
25.3 (20.2-31.9)
38.9 (37.0-40.0)
0.04; 0.02
18.9 (18.4-23.5)
0.02; 0.03
28.7 (22.9-30.7)
Fat free mass, kg
53.2 (51.4-68.6)
51.0 (49.6-59.5)
45.1 (37.6-46.4)
0.03; 0.03
55.1 (52.4-57.9)
Fat free mass index,
kg/m2 18.2 (16.9-19.4) 17.3 (16.2-19.1) 0.04; 0.04 15.6 (14.5-16.9) 0.04; 0.04 19.3 (18.3-20.6) 0.07

*Significance of differences calculated with the Mann-Whitney test.

P value of differences with the group of patients with normal BMI.

P value of differences with the control group.

[TableWrap ID: T3] Table 3 

Serum concentration of adiponectin, interleukin-6, and vascular endothelial growth factor in study patients*

Parameter(median and range) Control group (n = 20) Patients with overweight and obesity (n = 26) P Underweight patients
(n = 18) P Patients with normal body mass index (n = 39) P
Adiponectin, μg/mL
6.8 (3-7.1)
2.1 (0.8-3.9)
0.01; 0.03
12.7 (9.3-14.8)
0.03; 0.01
8.9 (7.2-11.3)
Interleukin-6, pg/mL
5.0 (2.3-9.9)
57.3 (50.6-73.3)
†0.03; 0001
25.2 (18.8-32.9)
0.03; 0.001
42.4 (29.3-53.9)
Vascular endothelial growth factor, pg/mL 20.0 (12.3-39.4) 175.0 (171.8-203.9) †0.02; 0.002 119.0 (108.1-169.2) 0.02 111 (73.1-158) 0.02

*Significance of differences calculated with the Mann-Whitney test.

P value of differences with the group of patients with normal BMI.

P value of differences with the control group.

Article Categories:
  • Clinical Science

Previous Document:  Cardiovascular effect of nifedipine in morphine dependent rats: hemodynamic, histopathological, and ...
Next Document:  Assessment of Turkish junior male physicians' exposure to mobbing behavior.