Document Detail


Boar sperm encapsulation reduces in vitro polyspermy.
MedLine Citation:
PMID:  19144017     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A boar sperm encapsulation technology in barium alginate has been developed to enhance reproductive performances and spermatozoa preservation time; aim of this work was to evaluate the effect of in vitro sperm encapsulation on polyspermy as a function of storage time at 18 degrees C. A total number of 40 in vitro fertilization (IVF) tests were performed using encapsulated or diluted spermatozoa (20 IVF each treatment). Overall, 1288 in vitro matured oocytes were fertilized with spermatozoa stored at 24, 48 or 72 h at 18 degrees C for both treatments polyspermy and normospermy, and the non-penetration rates were assessed by optical microscopy. Results indicate a significant reduction in risk of polyspermic oocytes when spermatozoa are preserved in barium alginate membranes (incidence risk ratio: 0.766 with respect to diluted); such enhancement could be explained by lesser damage of sperm membranes achieved by encapsulation technology.
Authors:
M Faustini; M Bucco; G Galeati; M Spinaci; S Villani; T Chlapanidas; I Ghidoni; D Vigo; M L Torre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-19
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  45     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-07-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  359-62     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy. massimo.faustini@unimi.it
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