Document Detail

Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions.
MedLine Citation:
PMID:  15823546     Owner:  NLM     Status:  MEDLINE    
In mammals, trefoil factor family (TFF) proteins are involved in mucosal maintenance and repair, and they are also implicated in tumor suppression and cancer progression. A novel two domain TFF protein from frog Bombina maxima skin secretions (Bm-TFF2) has been purified and cloned. It activated human platelets in a dose-dependent manner and activation of integrin alpha(IIb)beta(3) was involved. Aspirin and apyrase did not largely reduce platelet response to Bm-TFF2 (a 30% inhibition), indicating that the aggregation is not substantially dependent on ADP and thromboxane A2 autocrine feedback. Elimination of external Ca(2+) with EGTA did not influence the platelet aggregation induced by Bm-TFF2, meanwhile a strong calcium signal (cytoplasmic Ca(2+) release) was detected, suggesting that activation of phospholipase C (PLC) is involved. Subsequent immunoblotting revealed that, unlike in platelets activated by stejnulxin (a glycoprotein VI agonist), PLCgamma2 was not phosphorylated in platelets activated by Bm-TFF2. FITC-labeled Bm-TFF2 bound to platelet membranes. Bm-TFF2 is the first TFF protein reported to possess human platelet activation activity.
Jie Zhang; Yong Zhang; Shao-Gui Wan; Shuang-Shuang Wei; Wen-Hui Lee; Yun Zhang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  330     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1027-33     Citation Subset:  IM    
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
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MeSH Terms
Amino Acid Sequence
Calcium / metabolism
Cell Membrane / metabolism
Molecular Sequence Data
Mucins / chemistry,  isolation & purification*,  pharmacology*
Muscle Proteins / chemistry,  isolation & purification*,  pharmacology*
Peptides / chemistry,  isolation & purification*,  pharmacology*
Platelet Activation / drug effects*
Protein Binding
Skin / secretion
Reg. No./Substance:
0/Mucins; 0/Muscle Proteins; 0/Peptides; 146046-78-8/trefoil factor; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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