| Blunt chest trauma induces mediator-dependent monocyte migration to the lung. | |
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MedLine Citation:
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PMID: 20543668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: This study was designed to determine whether lung contusion induces an increased pulmonary recruitment of monocytes as a source of alveolar macrophages and which mediators are involved. SETTING AND DESIGN: Prospective animal study. SUBJECTS AND INTERVENTIONS: Male Sprague-Dawley rats were subjected to chest trauma by a single blast wave. MEASUREMENTS: Chemokine concentrations in bronchoalveolar lavage fluids and supernatants of alveolar macrophages, chemokine and chemokine receptor mRNA expressions in monocytes, pulmonary interstitial macrophages, and alveolar macrophages isolated after trauma or sham procedure were evaluated. Immigration of monocytes was determined by staining alveolar macrophages with the fluorescent marker PKH26 before chest trauma. Chemotaxis of naïve monocytes in response to bronchoalveolar lavage or supernatants from alveolar macrophages isolated after trauma or sham procedure and the migratory response of monocytes isolated after trauma/sham to recombinant chemokines were measured. MAIN RESULTS: Chemokine levels in bronchoalveolar lavage and alveolar macrophage supernatants and the percentage of monocytes migrated to the lungs were increased after chest trauma. Lung contusion enhanced the mRNA expression for CCR2 in monocytes and interstitial macrophages and for monocyte chemotactic protein-1 in alveolar macrophages. Migration of naïve monocytes vs. bronchoalveolar lavage or alveolar macrophage supernatants from traumatized animals was increased when compared with samples from shams. Monocytes isolated 2 hrs after trauma showed a reduced migration to CINC-1 or monocyte chemotactic protein-1 compared with sham. CONCLUSIONS: Alveolar macrophages seem to contribute to increased chemokine concentrations in alveoli of animals subjected to blunt chest trauma. Mediators released by alveolar macrophage are potent stimuli for monocyte migration. Monocytes alter their chemokine receptor expression and are recruited to the lungs. |
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Authors:
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Daniel H Seitz; Ulrike Niesler; Annette Palmer; Michael Sulger; Sonja T Braumüller; Mario Perl; Florian Gebhard; Markus W Knöferl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Critical care medicine Volume: 38 ISSN: 1530-0293 ISO Abbreviation: Crit. Care Med. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-20 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0355501 Medline TA: Crit Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 1852-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Trauma Surgery, Hand, Plastic and Reconstructive Surgery University of Ulm, Ulm, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Bronchoalveolar Lavage Fluid Cell Movement* Chemokines / metabolism DNA Primers Enzyme-Linked Immunosorbent Assay Flow Cytometry Lung / immunology, pathology* Macrophages, Alveolar / immunology Male Monocytes / immunology* Prospective Studies RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Receptors, Chemokine / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Thoracic Injuries / immunology*, metabolism Wounds, Nonpenetrating / immunology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/DNA Primers; 0/RNA, Messenger; 0/Receptors, Chemokine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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