| Blueberry antagonism of C-2 ceramide disruption of Ca2+ responses and recovery in MAChR-transfected COS-7 cell. | |
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MedLine Citation:
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PMID: 18997296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Research indicates that muscarinic receptors (MAChRs) may show selective declines in sensitivity during aging and Alzheimer's disease (AD), and these decrements may be related to oxidative stress sensitivity. M1AChR - transfected COS-7 cells exhibit greater decrements in Ca;{2+} buffering following oxotremorine-induced depolarization than M3 cells. This loss, induced via dopamine (DA) or amyloid-beta (Abeta), is similar to those reported in many studies with respect to aging and AD, but were antagonized by blueberry (BB) treatment. However, they may be exacerbated by ceramide which shows considerable increases in AD and aging. Thus, the effects of DA, Abeta_{42} and/or BB were assessed on calcium parameters (e.g., calcium buffering) in the presence or absence of ceramide in M1 and M3 transfected cells. Results indicated that while ceramide did not produce profound deficits in calcium buffering, in either M1- or M3-transfected COS-7 cells, decrements were seen in the ability of M1- and M3-transfected cells to initially respond to oxotremorine, regardless of DA or Abeta_{42} exposure. BB pre-treatment (prior to ceramide) antagonized the ceramide treatment in M1-transfected cells but not M3-transfected cells, where ceramide suppressed BB effects. Results are discussed in terms of the effects on lipid induced changes in plasma membranes. |
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Authors:
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James A Joseph; Anna Neuman; Donna F Bielinski; Derek R Fisher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Alzheimer's disease : JAD Volume: 15 ISSN: 1387-2877 ISO Abbreviation: J. Alzheimers Dis. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-10 Completed Date: 2009-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9814863 Medline TA: J Alzheimers Dis Country: Netherlands |
Other Details:
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Languages: eng Pagination: 429-41 Citation Subset: IM |
Affiliation:
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USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., Boston, MA 02111, USA. james.joseph@ars.usda.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Protein
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metabolism Animals Blueberry Plant / chemistry* COS Cells Calcium / metabolism Calcium Signaling / drug effects* Ceramides / antagonists & inhibitors*, pharmacology* Cercopithecus aethiops Data Interpretation, Statistical Dopamine / pharmacology Oxidative Stress / drug effects Phenols / pharmacology* Receptors, Muscarinic / genetics* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Protein; 0/Ceramides; 0/Phenols; 0/Receptors, Muscarinic; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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