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Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells.
MedLine Citation:
PMID:  25231366     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces 'chloroplast de-anchoring', a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de-anchoring is known induced within one minute of irradiation with high-fluence-rate blue light specifically, preceding the commencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response. In search of the responsible photoreceptors, immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-kD exist in the plasma-membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120-kD polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner. The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologues (Vallisneria PHOTOTROPIN1 and 2) expressed in leaves were isolated. We propose that calcium-regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisnera.
Authors:
Yuuki Sakai; Shin-Ichiro Inoue; Akiko Harada; Ken-Ichiro Shimazaki; Shingo Takagi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-17
Journal Detail:
Title:  Journal of integrative plant biology     Volume:  -     ISSN:  1744-7909     ISO Abbreviation:  J Integr Plant Biol     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101250502     Medline TA:  J Integr Plant Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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