| The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis. | |
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MedLine Citation:
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PMID: 12444098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase-negative immortalized human cells maintain telomeres by alternative lengthening of telomeres (ALT) pathway(s), which may involve homologous recombination. We find that endogenous BLM protein co-localizes with telomeric foci in ALT human cells but not telomerase positive immortal cell lines or primary cells. BLM interacts in vivo with the telomeric protein TRF2 in ALT cells, as detected by FRET and co-immunoprecipitation. Transient over-expression of green fluorescent protein (GFP)-BLM results in marked, ALT cell-specific increases in telomeric DNA. The association of BLM with telomeres and its effect on telomere DNA synthesis require a functional helicase domain. Our results identify BLM as the first protein found to affect telomeric DNA synthesis exclusively in human ALT cells and suggest that BLM facilitates recombination-driven amplification of telomeres in ALT cells. |
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Authors:
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Dimitrios J Stavropoulos; Paul S Bradshaw; Xiaobin Li; Ivan Pasic; Kevin Truong; Mitsuhiko Ikura; Mark Ungrin; M Stephen Meyn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human molecular genetics Volume: 11 ISSN: 0964-6906 ISO Abbreviation: Hum. Mol. Genet. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-21 Completed Date: 2003-05-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9208958 Medline TA: Hum Mol Genet Country: England |
Other Details:
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Languages: eng Pagination: 3135-44 Citation Subset: IM |
Affiliation:
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Department of Molecular and Medical Genetics, University of Toronto, Toronto, Canada M5S 1A8. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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biosynthesis,
immunology,
metabolism* Bloom Syndrome / enzymology*, genetics* Cell Line Cell Line, Transformed Cell Nucleus DNA / biosynthesis* DNA Helicases / biosynthesis, immunology, metabolism* Fibroblasts / chemistry, enzymology, virology Green Fluorescent Proteins Humans Luminescent Proteins / biosynthesis, genetics RecQ Helicases Recombinant Proteins / biosynthesis, genetics Telomere / enzymology, genetics, metabolism* Telomeric Repeat Binding Protein 2 / immunology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/Recombinant Proteins; 0/Telomeric Repeat Binding Protein 2; 147336-22-9/Green Fluorescent Proteins; 9007-49-2/DNA; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/Bloom syndrome protein; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/RecQ Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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