| The Bloom syndrome protein limits the lethality associated with RAD51 deficiency. | |
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MedLine Citation:
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PMID: 20215422 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Little is known about the functional interaction between the Bloom's syndrome protein (BLM) and the recombinase RAD51 within cells. Using RNA interference technology, we provide the first demonstration that RAD51 acts upstream from BLM to prevent anaphase bridge formation. RAD51 downregulation was associated with an increase in the frequency of BLM-positive anaphase bridges, but not of BLM-associated ultrafine bridges. Time-lapse live microscopy analysis of anaphase bridge cells revealed that BLM promoted cell survival in the absence of Rad51. Our results directly implicate BLM in limiting the lethality associated with RAD51 deficiency through the processing of anaphase bridges resulting from the RAD51 defect. These findings provide insight into the molecular basis of some cancers possibly associated with variants of the RAD51 gene family. |
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Authors:
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Kenza Lahkim Bennani-Belhaj; Sébastien Rouzeau; Géraldine Buhagiar-Labarchède; Pauline Chabosseau; Rosine Onclercq-Delic; Emilie Bayart; Fabrice Cordelières; Jérôme Couturier; Mounira Amor-Guéret |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-09 |
Journal Detail:
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Title: Molecular cancer research : MCR Volume: 8 ISSN: 1557-3125 ISO Abbreviation: Mol. Cancer Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-18 Completed Date: 2010-07-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101150042 Medline TA: Mol Cancer Res Country: United States |
Other Details:
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Languages: eng Pagination: 385-94 Citation Subset: IM |
Affiliation:
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Institut Curie, Centre de Recherche, Centre Universitaire, Orsay, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaphase
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genetics Cell Death / genetics Cell Survival / genetics Down-Regulation / genetics Gene Expression Regulation, Neoplastic / genetics Hela Cells Humans Neoplasms / genetics*, metabolism RNA Interference / physiology Rad51 Recombinase / genetics* RecQ Helicases / genetics*, metabolism Sister Chromatid Exchange / genetics |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.-/Rad51 Recombinase; EC 3.6.1.-/Bloom syndrome protein; EC 3.6.1.-/RecQ Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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