Document Detail

Blood volume assessment with hemoglobin subtype analysis in preterm infants.
MedLine Citation:
PMID:  12890935     Owner:  NLM     Status:  MEDLINE    
The aim of the study was to assess plasma volume (PV) and red cell volume (RCV) in preterm infants with a liquid chromatographic hemoglobin subtype method (HbST) based on dilution of autologous fetal hemoglobin (HbF) by donor adult hemoglobin and measurement of the amount of HbF. PV determination with the indocyanine green dye dilution technique (ICG) was used as a reference method. Eight infants, median gestational age 29.8 weeks (range 27.6-30.9 weeks) and median birth weight 1,300 g (range 1,030-1,760 g), were studied at a median age of 3.0 days (range 1-6 days). RCV was 33.6 +/- 12 ml/kg measured with ICG and 32.1 +/- 5.2 ml/kg with HbST. PV was 47.0 +/- 18 and 40.2 +/- 6.6 ml/kg, respectively. There was a close correlation between the RCVs measured with the two techniques (Pearson correlation 0.83, p < 0.05). In conclusion, HbST provides a reliable and safe determination of RCV in preterm infants.
Jaana A Leipälä; Marjo Talme; Juha Viitala; Ursula Turpeinen; Vineta Fellman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the neonate     Volume:  84     ISSN:  0006-3126     ISO Abbreviation:  Biol. Neonate     Publication Date:  2003  
Date Detail:
Created Date:  2003-07-31     Completed Date:  2004-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0247551     Medline TA:  Biol Neonate     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Hospital for Children and Adolescents, University of Helsinki, Finland.
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MeSH Terms
Birth Weight
Chromatography, High Pressure Liquid
Erythrocyte Transfusion
Erythrocyte Volume*
Fetal Hemoglobin / analysis
Gestational Age
Hemoglobin A
Hemoglobins / analysis*
Indicator Dilution Techniques
Indocyanine Green
Infant, Newborn
Infant, Premature*
Plasma Volume*
Reg. No./Substance:
0/Hemoglobins; 3599-32-4/Indocyanine Green; 9034-51-9/Hemoglobin A; 9034-63-3/Fetal Hemoglobin

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