Document Detail

Blood storage XXIII: 2,3-DPG maintenance for six weeks in a CPD-adenine-inosine preservative with and without methylene blue.
MedLine Citation:
PMID:  867464     Owner:  NLM     Status:  MEDLINE    
In a pilot study the optimal concentration of inosine for 2,3-DPG maintenance in a CPD-adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3-DPG maintenance was nearly normal for six weeks of storage in CPD-adenine-inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3-DPG concentrations after the 3rd week. Finally, 2,3-DPG levels were significantly better maintained in CPD-adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(-6)M), compared to CPD-adenine-inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3-DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3-DPG for prolonged (five to six weeks) liquid storage.
R B Dawson
Related Documents :
1000574 - Morphometric in vitro analysis of the control of the activity of the neurosecretory dar...
10168554 - The storage quality of apheresis platelets--analysis of results from seven different ce...
7660164 - Quality control of platelet concentrates by the thrombostat 4000.
5540174 - Platelet storage at 22 degrees c; metabolic, morphologic, and functional studies.
8329564 - Electrophoretic light scattering studies on the interaction of fibrinogen with resting ...
16793654 - Genetic control of platelet activation in inbred mouse strains.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  17     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:    1977 May-Jun
Date Detail:
Created Date:  1977-07-23     Completed Date:  1977-07-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  238-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine Triphosphate / analysis
Analysis of Variance
Blood Preservation / methods,  standards*
Diphosphoglyceric Acids / analysis*
Methylene Blue
Reg. No./Substance:
0/Citrates; 0/Diphosphoglyceric Acids; 0/Phosphates; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 58-63-9/Inosine; 61-73-4/Methylene Blue; 73-24-5/Adenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  How donors and nondonors view people who do not give blood.
Next Document:  Blood storage XXIV: red blood cell 2,3-DPG and ATP maintenance for six weeks in CPD-adenine with hig...