Document Detail


Blood storage XXIII: 2,3-DPG maintenance for six weeks in a CPD-adenine-inosine preservative with and without methylene blue.
MedLine Citation:
PMID:  867464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a pilot study the optimal concentration of inosine for 2,3-DPG maintenance in a CPD-adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3-DPG maintenance was nearly normal for six weeks of storage in CPD-adenine-inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3-DPG concentrations after the 3rd week. Finally, 2,3-DPG levels were significantly better maintained in CPD-adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(-6)M), compared to CPD-adenine-inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3-DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3-DPG for prolonged (five to six weeks) liquid storage.
Authors:
R B Dawson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  17     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:    1977 May-Jun
Date Detail:
Created Date:  1977-07-23     Completed Date:  1977-07-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  238-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenine
Adenosine Triphosphate / analysis
Analysis of Variance
Blood Preservation / methods,  standards*
Citrates
Diphosphoglyceric Acids / analysis*
Glucose
Humans
Inosine
Methylene Blue
Phosphates
Chemical
Reg. No./Substance:
0/Citrates; 0/Diphosphoglyceric Acids; 0/Phosphates; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 58-63-9/Inosine; 61-73-4/Methylene Blue; 73-24-5/Adenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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