| Blood separation on microfluidic paper-based analytical devices. | |
| | |
MedLine Citation:
|
PMID: 22782449 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A microfluidic paper-based analytical device (μPAD) for the separation of blood plasma from whole blood is described. The device can separate plasma from whole blood and quantify plasma proteins in a single step. The μPAD was fabricated using the wax dipping method, and the final device was composed of a blood separation membrane combined with patterned Whatman No.1 paper. Blood separation membranes, LF1, MF1, VF1 and VF2 were tested for blood separation on the μPAD. The LF1 membrane was found to be the most suitable for blood separations when fabricating the μPAD by wax dipping. For blood separation, the blood cells (both red and white) were trapped on blood separation membrane allowing pure plasma to flow to the detection zone by capillary force. The LF1-μPAD was shown to be functional with human whole blood of 24-55% hematocrit without dilution, and effectively separated blood cells from plasma within 2 min when blood volumes of between 15-22 μL were added to the device. Microscopy was used to confirm that the device isolated plasma with high purity with no blood cells or cell hemolysis in the detection zone. The efficiency of blood separation on the μPAD was studied by plasma protein detection using the bromocresol green (BCG) colorimetric assay. The results revealed that protein detection on the μPAD was not significantly different from the conventional method (p > 0.05, pair t-test). The colorimetric measurement reproducibility on the μPAD was 2.62% (n = 10) and 5.84% (n = 30) for within-day and between day precision, respectively. Our proposed blood separation on μPAD has the potential for reducing turnaround time, sample volume, sample preparation and detection processes for clinical diagnosis and point-of care testing. |
| | |
Authors:
|
Temsiri Songjaroen; Wijitar Dungchai; Orawon Chailapakul; Charles S Henry; Wanida Laiwattanapaisal |
Related Documents
:
|
20361259 - Partitioned fluid-solid coupling for cardiovascular blood flow: validation study of pre... 10532609 - Effects of granulocyte-colony-stimulating factor on potential normal granulocyte donors. 1508889 - Extracellular fluid movement in the pulp; the pulp/dentin permeability barrier. 22401759 - Contributions of dynamic venous blood volume versus oxygenation level changes to bold f... 11114579 - An in vitro bacterial touch contamination risk assessment of two capd twinbag systems. 8331719 - Contribution of increased cerebral blood volume to posttraumatic intracranial hypertens... 975949 - Reduction of blood usage in open heart surgery. 1150509 - Evaluation of harleco co2 apparatus: comparison with the van slyke method. 7754839 - Effects of antioxidants on the blood-brain barrier and postischemic hyperemia. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-7-10 |
Journal Detail:
|
Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-7-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Patumwan, Bangkok, 10330, Thailand. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tendoscopic treatment of recurrent peroneal tendon dislocation.
Next Document: Rational diagnostic strategies for Lyme borreliosis in children and adolescents: recommendations by ...