| Blood plasma contact activation on silicon, titanium and aluminium. | |
| | |
MedLine Citation:
|
PMID: 17156838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the present work, blood plasma protein deposition to spontaneously air oxidized silicon, titanium and aluminium was re-investigated in vitro. Immunological- and null ellipsometry methods were used to detect and quantitate adsorbed proteins, RIA methods to study the retention of preadsorbed 125I-HSA upon exposure to buffer or blood plasma, and kallikrein-specific colorimetric substrate S-2302 to follow the surface generation of kallikrein. The results show that the contact activation of coagulation and complement systems are connected on Si and Ti, but not on Al, via coagulation factor XII. Preadsorbed 125I-HSA was most readily displaced on silicon, followed by titanium and aluminium. The surfaces displayed different antibody binding patterns after short and long-time exposures to plasma. Titanium and silicon bound anti-HMWK after 1 min in plasma, but aluminium did not. When the plasma incubation time was prolonged up to 2h the anti-HMWK binding disappeared totally on titanium and decreased on silicon. During the same time period, anti-C3c binding increased to the three types of surfaces. Also, the anti-C3c binding onto Si and Ti, but not Al, disappeared after incubation in Factor XII deficient plasma or when a specific coagulation factor XII (Factor XII) inhibitor, corn trypsin inhibitor (CTI) was added to normal plasma. The surface contacted plasmas cleaved the kallikrein-specific reagent S-2302 both after single surface contact, and after reincubation of surfaces in fresh plasma. The results show that C3b and Factor XIIa and their degradation products were retained at the surfaces. |
| | |
Authors:
|
Sara Arvidsson; Agneta Askendal; Pentti Tengvall |
Related Documents
:
|
3849928 - The kallikrein-kinin system in operated and non-operated patients with acute pancreatitis. 16728058 - Dynamics of exogenous kallikrein-stimulated bovine sperm motility in the presence of se... 1737048 - The major plasma kallikrein inhibitor of guinea pig plasma. 1312788 - Role of brain kallikrein-kinin system in regulation of adrenocorticotropin release. 1554198 - Comparison of elastin peptide concentrations in body fluids from healthy volunteers, sm... 9389898 - Influence of malaria on markers of iron status in children: implications for interpreti... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-06 |
Journal Detail:
|
Title: Biomaterials Volume: 28 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2006-12-25 Completed Date: 2007-03-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 1346-54 Citation Subset: IM |
Affiliation:
|
Division of Applied Physics, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Aluminum Biocompatible Materials* Blood Coagulation Blood Proteins / metabolism Buffers Colorimetry Complement Activation Heparin Humans Iodine Radioisotopes Kallikreins / biosynthesis Materials Testing Plasma / metabolism* Protein Binding Serum Albumin / metabolism Silicon Surface Properties Titanium |
| Chemical | |
Reg. No./Substance:
|
0/Biocompatible Materials; 0/Blood Proteins; 0/Buffers; 0/Iodine Radioisotopes; 0/Serum Albumin; 7429-90-5/Aluminum; 7440-21-3/Silicon; 7440-32-6/Titanium; 9005-49-6/Heparin; EC 3.4.21.-/Kallikreins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Self-assembled ternary complexes of plasmid DNA, low molecular weight polyethylenimine and targeting...
Next Document: Dual effects of cyclic ADP-ribose on sarcoplasmic reticulum Ca2+ release and storage in cardiac myoc...