Document Detail

Blood-perfused working isolated rat heart.
MedLine Citation:
PMID:  985409     Owner:  NLM     Status:  MEDLINE    
We describe a method for perfusion of a working isolated rat heart with washed erythrocytes suspended in a Krebs-Henseleit bicarbonate buffer containing bovine albumin (fraction V). With washed pig red cells, as hematocrit was varied between 0 and 40%, coronary flow (CF), aortic flow (AF), external work (W), and myocardial oxygen consumption (MVO2) were measured. Hemodynamic data at a hematocrit of 30% (CF = 5.4 +/- 0.7 ml/min per g, AF = 75 +/- 8 ml/min per g) were identical with those reported for the intact animal. Coronary sinus PO2 was highest with a red cell-free perfusate suggesting that coronary flow is partially shunted. Human red cells obtained from banked blood, were tried also with success. With careful filtration, the preparation is stable for 2 h and well suited for study of the dynamics of myocardial oxygen delivery.
M A Duvelleroy; M Duruble; J L Martin; B Teisseire; J Droulez; M Cain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied physiology     Volume:  41     ISSN:  0021-8987     ISO Abbreviation:  J Appl Physiol     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1976-12-23     Completed Date:  1976-12-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376576     Medline TA:  J Appl Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  603-7     Citation Subset:  IM    
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MeSH Terms
Heart / physiology*
Oxygen Consumption*
Plasma Substitutes*
Reg. No./Substance:
0/Plasma Substitutes

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