| Blood monocytes from most human immunodeficiency virus type 1-infected patients do not carry proviral DNA. | |
| | |
MedLine Citation:
|
PMID: 8556497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In blood, the CD4+ T cells of patients with human immunodeficiency virus type 1 (HIV-1) harbor HIV-1; however, whether the CD4+ blood monocytes carry the virus is controversial. Tissue macrophages are known to be infected. To determine in blood monocytes from HIV-1-seropositive patients contain HIV-1, we separated monocytes and T-cell subsets by using monoclonal antibodies bound to magnetic beads and by monocyte adherence to glass. Monocytes were cultured with macrophage colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and interleukin-3. After 14 days in culture, cells were analyzed for the presence of HIV-1 antigen and multinucleated giant cells (MGCs). Freshly isolated cell subsets were analyzed for HIV-1 proviral DNA by PCR with modified env (SK68i and SK69i2) and gag (SK145i and SK150) primers. We found that (i) monocytes cultured without depletion of CD4+ T cells (11 of 11 patients) were HIV-1 antigen positive and showed dramatically increased spontaneous formation of MGCs (ii) monocytes cultured after depletion of CD4+ T cells (three experiments) were HIV-1 antigen negative and showed markedly decreased MGC formation, and (iii) in specimens from 14 patients subsequently analyzed by PCR, purified CD4+ T cells were positive for HIV-1 proviral DNA in all patients. In 11 of 14 patients (79%), the monocyte fractions were HIV-1 proviral DNA negative, while in the remaining 3 patients, the monocytes were positive for HIV-1 proviral DNA. In conclusion, the major reservoir for HIV-1 infection in human peripheral blood is the CD4+ T cell (14 of 14 cases).(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
Y Shen; J Rudnik; S Cassol; J Drouin; W Cameron; C A Izaguirre; L G Filion |
Related Documents
:
|
7903507 - Transfection of a retroviral construct carrying a non producer hiv-1 variant induces hi... 1915247 - A comparative analysis of alterations in protein expression after activation or human i... 1406627 - The plant amino acid mimosine may inhibit initiation at origins of replication in chine... 10559367 - Consistent viral evolutionary changes associated with the progression of human immunode... 15958707 - Thrombopoietin and the hematopoietic stem cell. 17920947 - Phenotypically and functionally distinct subsets of natural killer cells in human pbmcs. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical and diagnostic laboratory immunology Volume: 1 ISSN: 1071-412X ISO Abbreviation: Clin. Diagn. Lab. Immunol. Publication Date: 1994 Sep |
Date Detail:
|
Created Date: 1996-02-23 Completed Date: 1996-02-23 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 9421292 Medline TA: Clin Diagn Lab Immunol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 531-7 Citation Subset: IM; X |
Affiliation:
|
Department of Microbiology, University of Ottawa General Hospital, Ontario, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acquired Immunodeficiency Syndrome
/
blood* Antigens, Viral / immunology Base Sequence CD4-Positive T-Lymphocytes / immunology, virology CD8-Positive T-Lymphocytes / immunology, virology Cells, Cultured / immunology, virology DNA, Viral / analysis HIV-1 / genetics, immunology* Humans Magnetics Microspheres Molecular Sequence Data Monocytes / immunology, virology* Polymerase Chain Reaction T-Lymphocyte Subsets / immunology, virology |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Viral; 0/DNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Measurement of pneumococcal capsular polysaccharide serotype-specific immunoglobulin G in human seru...
Next Document: Interleukin-2-inducible natural immune (lymphokine-activated killer cell) responses as a functional ...