Document Detail

Blood monocytes from most human immunodeficiency virus type 1-infected patients do not carry proviral DNA.
MedLine Citation:
PMID:  8556497     Owner:  NLM     Status:  MEDLINE    
In blood, the CD4+ T cells of patients with human immunodeficiency virus type 1 (HIV-1) harbor HIV-1; however, whether the CD4+ blood monocytes carry the virus is controversial. Tissue macrophages are known to be infected. To determine in blood monocytes from HIV-1-seropositive patients contain HIV-1, we separated monocytes and T-cell subsets by using monoclonal antibodies bound to magnetic beads and by monocyte adherence to glass. Monocytes were cultured with macrophage colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and interleukin-3. After 14 days in culture, cells were analyzed for the presence of HIV-1 antigen and multinucleated giant cells (MGCs). Freshly isolated cell subsets were analyzed for HIV-1 proviral DNA by PCR with modified env (SK68i and SK69i2) and gag (SK145i and SK150) primers. We found that (i) monocytes cultured without depletion of CD4+ T cells (11 of 11 patients) were HIV-1 antigen positive and showed dramatically increased spontaneous formation of MGCs (ii) monocytes cultured after depletion of CD4+ T cells (three experiments) were HIV-1 antigen negative and showed markedly decreased MGC formation, and (iii) in specimens from 14 patients subsequently analyzed by PCR, purified CD4+ T cells were positive for HIV-1 proviral DNA in all patients. In 11 of 14 patients (79%), the monocyte fractions were HIV-1 proviral DNA negative, while in the remaining 3 patients, the monocytes were positive for HIV-1 proviral DNA. In conclusion, the major reservoir for HIV-1 infection in human peripheral blood is the CD4+ T cell (14 of 14 cases).(ABSTRACT TRUNCATED AT 250 WORDS)
Y Shen; J Rudnik; S Cassol; J Drouin; W Cameron; C A Izaguirre; L G Filion
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  1     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1996-02-23     Completed Date:  1996-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  531-7     Citation Subset:  IM; X    
Department of Microbiology, University of Ottawa General Hospital, Ontario, Canada.
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MeSH Terms
Acquired Immunodeficiency Syndrome / blood*
Antigens, Viral / immunology
Base Sequence
CD4-Positive T-Lymphocytes / immunology,  virology
CD8-Positive T-Lymphocytes / immunology,  virology
Cells, Cultured / immunology,  virology
DNA, Viral / analysis
HIV-1 / genetics,  immunology*
Molecular Sequence Data
Monocytes / immunology,  virology*
Polymerase Chain Reaction
T-Lymphocyte Subsets / immunology,  virology
Reg. No./Substance:
0/Antigens, Viral; 0/DNA, Viral

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