| Blood monitoring of granzyme B and perforin expression after intestinal transplantation: considerations on clinical relevance. | |
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MedLine Citation:
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PMID: 18580471 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The use of biomarkers for rejection monitoring represents a major goal in intestinal transplantation. We analyzed the blood expression of Granzyme B (GB) and Perforin (PF) in the following pathological conditions after intestinal transplantation: acute rejection (AR), Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection, and posttransplant lymphoproliferative disease (PTLD). The diagnostic accuracy and the clinical utility of these tests are finally discussed. METHODS: GB and PF levels were measured by real time polymerase chain reaction on peripheral blood samples from 32 intestinal recipients. Blood samples (n=494) after comparison of clinical, histological, and microbiological data were assigned to the following groups: normal (n=307), AR (n=30), EBV infection (n=107), CMV infection (n=25), and PTLD (n=25). RESULTS: Mean levels of GB and PF in the AR (GB=279.7; PF=256.7), PTLD (GB=199; PF=185.9), EBV (GB=133.2; PF=143.7), and CMV (GB=151.3; PF=144) groups were significantly higher than in the normal group (GB=100.1; PF=101.1) (all P<0.05, except for PF in CMV infection). The best accuracy was obtained for the diagnosis of AR with sensitivity and specificity of 80% and 79% for GB and 70% and 79% for PF, respectively. The area under the receiver-operator characteristics curve was 0.87 for GB and 0.82 for PF. CONCLUSIONS: GB and PF are diagnostic molecular markers of AR. GB and PF blood levels are also increased in case of viral infections or PTLD. Serial blood testing for GB and PF might be predictive of early intestinal graft dysfunction and should be interpreted in the context of the histological and virological analyses. |
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Authors:
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Annalisa Altimari; Elisa Gruppioni; Elisa Capizzi; Alberto Bagni; Barbara Corti; Michelangelo Fiorentino; Tiziana Lazzarotto; Augusto Lauro; Antonio Daniele Pinna; Lorenza Ridolfi; Walter Franco Grigioni; Antonia D'errico-Grigioni |
Publication Detail:
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Type: Journal Article; Multicenter Study |
Journal Detail:
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Title: Transplantation Volume: 85 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-26 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: United States |
Other Details:
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Languages: eng Pagination: 1778-83 Citation Subset: IM |
Affiliation:
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Pathology Unit, Istituto Oncologico "F. Addarii," S. Orsola-Malpighi Hospital, Bologna University Bologna, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Biological Markers / blood Cytomegalovirus Infections / blood, diagnosis* Epstein-Barr Virus Infections / blood, diagnosis* Follow-Up Studies Graft Rejection / blood, diagnosis* Granzymes / blood*, metabolism Humans Intestines / transplantation* Leukemia, Large Granular Lymphocytic Middle Aged Organ Transplantation Perforin / blood*, metabolism Postoperative Complications / blood, diagnosis, virology Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 126465-35-8/Perforin; EC 3.4.21.-/Granzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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