Document Detail


Blood lymphocyte chimerism associated with IVF and monochorionic dizygous twinning: case report.
MedLine Citation:
PMID:  15375077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report on dizygotic (DZ) twins, conceived by IVF and ICSI with assisted hatching, who each had a mixture of 46,XX and 46,XY cells in blood lymphocytes. The female twin had mild genitalia abnormalities but further study revealed anatomically normal reproductive anatomy. Chromosome and fluorescence in situ hybridization studies of buccal, skin and ovarian tissue were normal, as were buccal tissue DNA studies. Fetal ultrasound and fetal membrane pathology were consistent with a monochorionic, diamniotic placenta (MCDAP). These twins thus have blood chimerism but are not chimeric in the other tissues studied. The mechanism for the chimerism could be due to either placental vascular anastamoses (after the development of the haematoblast stem cells) or due to an admixture of trophoblast cells during early blastocyst development. Such trophoblast cell admixtures would be restricted to the extraembryonic tissues so that general physical development in the fetus is normal and without somatic cell chimerism. This case in combination with others previously reported suggests that in IVF conceptions, the prevalence of blood chimerism associated with twinning, and the occurrence of DZ twinning associated with MCDAP, may be higher than previously thought.
Authors:
C A Williams; M R Wallace; K C Drury; S Kipersztok; R K Edwards; R S Williams; M J Haller; D A Schatz; J H Silverstein; B A Gray; R T Zori
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Publication Detail:
Type:  Case Reports; Journal Article     Date:  2004-09-16
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  19     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  2816-21     Citation Subset:  IM    
Affiliation:
The R.C.Philips Unit, Division of Genetics, Department of Pediatrics, University of Florida College of Medicine, Gainesville, Florida, USA. Willicx@peds.ufl.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Chimera*
Chorion
Diseases in Twins / genetics
Endocrine System / metabolism
Female
Fertilization in Vitro*
Fibroblasts / physiology
Genitalia / abnormalities
Humans
In Situ Hybridization, Fluorescence
Infant
Infant, Newborn
Lymphocytes / physiology*
Male
Microsatellite Repeats
Mosaicism
Ovary / abnormalities
Pregnancy
Skin / cytology
Twins, Dizygotic / genetics*
Ultrasonography, Prenatal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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