Document Detail

Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a-b+) p phenotype: absence of blood group structures based on the globoseries.
MedLine Citation:
PMID:  8737255     Owner:  NLM     Status:  MEDLINE    
Total neutral glycolipid fractions were isolated from kidney and ureter tissue obtained at autopsy of an individual of the rare blood group A1 Le(a-b+) p. The amount of glycolipids isolated were 3.7 and 2.5 mg g-1 dry tissue weight for the kidney and ureter tissue, which is in the range of reference blood group P kidneys. Part of the kidney glycolipid fraction was subfractionated by HPLC. Glycolipid compounds were structurally characterized by thin-layer chromatography (chemical detection and immunostaining with monoclonal antibodies), proton NMR spectroscopy and mass spectrometry. Globotriaosyl- and globotetraosyl-ceramides, which are the major compounds in kidneys of P individuals, were absent in the p kidney, and a comparatively increased amount of monoglycosyl- and lactosylceramides was found. A shift to longer fatty acyl chains in the ceramide part of lactosylceramides was noted. Elongated globoseries compounds with five to seven sugar residues, including the blood group A type 4 chain structure, were lacking. A slight increase in neolactotetraosyl- and blood group X pentaglycosyl-ceramides was noticed. The study confirms an enzymatic block in the conversion of lactosylceramide to elongated globoseries compounds in the kidney tissue similar to that of erythrocytes of p individuals.
K Lindström; P A Jovall; S Ghardashkani; B E Samuelsson; M E Breimer
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  13     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-10-16     Completed Date:  1996-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  307-13     Citation Subset:  IM    
Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska Hospital, Sweden.
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MeSH Terms
Antibodies, Monoclonal
Antibody Specificity
Blood Group Antigens / biosynthesis*,  chemistry
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Globosides / biosynthesis*,  chemistry
Glycolipids / biosynthesis*,  chemistry,  isolation & purification
Glycosphingolipids / biosynthesis*,  chemistry,  isolation & purification
Kidney / chemistry,  metabolism*
Magnetic Resonance Spectroscopy
Mass Spectrometry
P Blood-Group System / genetics*
Ureter / chemistry,  metabolism*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Blood Group Antigens; 0/Globosides; 0/Glycolipids; 0/Glycosphingolipids; 0/P Blood-Group System

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