Document Detail


Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a-b+) p phenotype: absence of blood group structures based on the globoseries.
MedLine Citation:
PMID:  8737255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Total neutral glycolipid fractions were isolated from kidney and ureter tissue obtained at autopsy of an individual of the rare blood group A1 Le(a-b+) p. The amount of glycolipids isolated were 3.7 and 2.5 mg g-1 dry tissue weight for the kidney and ureter tissue, which is in the range of reference blood group P kidneys. Part of the kidney glycolipid fraction was subfractionated by HPLC. Glycolipid compounds were structurally characterized by thin-layer chromatography (chemical detection and immunostaining with monoclonal antibodies), proton NMR spectroscopy and mass spectrometry. Globotriaosyl- and globotetraosyl-ceramides, which are the major compounds in kidneys of P individuals, were absent in the p kidney, and a comparatively increased amount of monoglycosyl- and lactosylceramides was found. A shift to longer fatty acyl chains in the ceramide part of lactosylceramides was noted. Elongated globoseries compounds with five to seven sugar residues, including the blood group A type 4 chain structure, were lacking. A slight increase in neolactotetraosyl- and blood group X pentaglycosyl-ceramides was noticed. The study confirms an enzymatic block in the conversion of lactosylceramide to elongated globoseries compounds in the kidney tissue similar to that of erythrocytes of p individuals.
Authors:
K Lindström; P A Jovall; S Ghardashkani; B E Samuelsson; M E Breimer
Related Documents :
8928955 - Thyroid, renal, and splanchnic circulation in horses at rest and during short-term exer...
6708565 - Glomerular permselection: shape and flow.
9652825 - Immunoglobulin prolongs survival of pig kidneys perfused ex vivo with human blood.
9020335 - Morphology of hdaf (cd55) transgenic pig kidneys following ex-vivo hemoperfusion with h...
22670125 - Massive bleeding and massive transfusion.
21835475 - Blunted pathogen-associated molecular pattern motif induced tnf, il-6 and il-10 product...
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  13     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-10-16     Completed Date:  1996-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  307-13     Citation Subset:  IM    
Affiliation:
Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska Hospital, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antibody Specificity
Blood Group Antigens / biosynthesis*,  chemistry
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Female
Globosides / biosynthesis*,  chemistry
Glycolipids / biosynthesis*,  chemistry,  isolation & purification
Glycosphingolipids / biosynthesis*,  chemistry,  isolation & purification
Humans
Kidney / chemistry,  metabolism*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Mice
P Blood-Group System / genetics*
Phenotype
Ureter / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Blood Group Antigens; 0/Globosides; 0/Glycolipids; 0/Glycosphingolipids; 0/P Blood-Group System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of neolactoglycolipids: sialosyl-, disialosyl-, O-acetyldisialosyl- and fucosyl- derivati...
Next Document:  Use of diethyl squarate for the coupling of oligosaccharide amines to carrier proteins and character...