| Blood group genotyping by high-throughput DNA analysis: application to the French panel of RBC reagents. | |
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MedLine Citation:
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PMID: 20655269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The use of blood group genotyping for the prediction of antigen expression has been discussed in clinical transfusion settings, but much less for reagent red blood cells selection. In France, the Centre National de Référence pour les Groupes Sanguins (CNRGS) produces a reference panel of reagent red blood cells, mainly used for red cell antibody identification. The use of high-throughput DNA analysis has never been applied to blood donors whose red blood cells are used as reagents. The aim of this study was to compare the serological phenotype and that predicted from DNA analysis in such donors, and to determine the benefit of DNA analysis in reagent red blood cells selection strategy. STUDY DESIGN AND METHOD: Red blood cells of 346 blood donors were typed with two different reagents for each antigen. The genotyping was performed by using HEA v1.2 BeadChips, BioArray Solutions, Immucor. The comparison between the serologically determined phenotype and that predicted from DNA analysis held on 8876 paired results obtained from 10 blood group systems and 25 antigens. RESULTS: A 99.95% concordance was observed. Four cases of discrepancy for RH, KEL, LU and DO blood group systems were analyzed. Genotyping precisions were of special interest for the Duffy blood group system. CONCLUSION: Systematic DNA analysis brings important information on reagent red blood cells selection. It can be used at a routine level. Especially, the notion of "antigen of double dose" which is specified in several countries by government bodies should evolve regarding data obtained from DNA analysis. This should improve the quality of reagent red blood cells as first step for antibody identification. |
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Authors:
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S Kappler-Gratias; T Peyrard; P Rouger; P-Y Le Pennec; B-N Pham |
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Publication Detail:
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Type: Journal Article Date: 2010-07-23 |
Journal Detail:
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Title: Transfusion clinique et biologique : journal de la Société française de transfusion sanguine Volume: 17 ISSN: 1953-8022 ISO Abbreviation: Transfus Clin Biol Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-23 Completed Date: 2011-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423846 Medline TA: Transfus Clin Biol Country: France |
Other Details:
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Languages: eng Pagination: 165-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Institut national de la transfusion sanguine, 6, rue Alexandre-Cabanel, 75015 Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Donors Blood Group Antigens / genetics*, isolation & purification DNA / blood*, genetics Erythrocytes / physiology France Genotype Humans Indicators and Reagents Phenotype |
| Chemical | |
Reg. No./Substance:
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0/Blood Group Antigens; 0/Indicators and Reagents; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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