| Blood genomic expression profile for neuronal injury. | |
| | |
MedLine Citation:
|
PMID: 12621306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study determined whether stroke and other types of insults produced a gene expression profile in blood that correlated with the presence of neuronal injury. Adult rats were subjected to ischemic stroke, intracerebral hemorrhage, status epilepticus, and insulin-induced hypoglycemia and compared with untouched, sham surgery, and hypoxia animals that had no brain injury. One day later, microarray analyses showed that 117 genes were upregulated and 80 genes were downregulated in mononuclear blood cells of the "injury" (n = 12) compared with the "no injury" (n = 9) animals. A second experiment examined the whole blood genomic response of adult rats after global ischemia and kainate seizures. Animals with no brain injury were compared with those with brain injury documented by TUNEL and PANT staining. One day later, microarray analyses showed that 37 genes were upregulated and 67 genes were downregulated in whole blood of the injury (n = 4) animals compared with the no-injury (n = 4) animals. Quantitative reverse transcription-polymerase chain reaction confirmed that the vesicular monoamine transporter-2 increased 2.3- and 1.6-fold in animals with severe and mild brain injury, respectively, compared with no-injury animals. Vascular tyrosine phosphatase-1 increased 2.0-fold after severe injury compared with no injury. The data support the hypothesis that there is a peripheral blood genomic response to neuronal injury, and that this blood response is associated with a specific blood mRNA gene expression profile that can be used as a marker of the neuronal damage. |
| | |
Authors:
|
Yang Tang; Alex C Nee; Aigang Lu; Ruiqiong Ran; Frank R Sharp |
Related Documents
:
|
7134006 - Radiation effects of iridium-192 implants in the cat brain. 17205016 - Hyperventilation following head injury: effect on ischemic burden and cerebral oxidativ... 7004596 - The "milieu interieur"-a model of brain physiology and pathophysiology. 14724226 - Brain uptake of the glucagon-like peptide-1 antagonist exendin(9-39) after intranasal a... 21412356 - In vitro production of transfusable red blood cells. 8804496 - Complications during transurethral vaporization of the prostate. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 23 ISSN: 0271-678X ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2003 Mar |
Date Detail:
|
Created Date: 2003-03-06 Completed Date: 2003-04-08 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
|
Languages: eng Pagination: 310-9 Citation Subset: IM |
Affiliation:
|
Department of Neurology, Neuroscience Program, University of Cincinnati, Vontz Center for Molecular Studies-Room 2327, 3125 Eden Avenue, Cincinnati, OH 45267-0536, USA. tangyy@email.uc.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Physiological Phenomena Brain Diseases / blood*, genetics* Down-Regulation Gene Expression Profiling* Genome* Ischemic Attack, Transient / pathology, physiopathology Kainic Acid Male Membrane Glycoproteins / genetics, metabolism Membrane Transport Proteins* Monocytes / physiology Neuropeptides* Oligonucleotide Array Sequence Analysis Protein Phosphatase 1 Protein Tyrosine Phosphatases / genetics RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Reference Values Reverse Transcriptase Polymerase Chain Reaction Seizures / chemically induced, pathology, physiopathology Vesicular Biogenic Amine Transport Proteins Vesicular Monoamine Transport Proteins |
| Grant Support | |
ID/Acronym/Agency:
|
AG 19561/AG/NIA NIH HHS; NS 28167/NS/NINDS NIH HHS; NS 38084/NS/NINDS NIH HHS; NS 38743/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Neuropeptides; 0/RNA, Messenger; 0/Vesicular Biogenic Amine Transport Proteins; 0/Vesicular Monoamine Transport Proteins; 487-79-6/Kainic Acid; EC 3.1.3.16/Protein Phosphatase 1; EC 3.1.3.48/Protein Tyrosine Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of dopamine loss and the metabolite 3-O-methyl-[18F]fluoro-dopa on the relation between the 1...
Next Document: Apaf-1, Bcl-xL, cytochrome c, and caspase-9 form the critical elements for cerebral vascular protect...