| Blood flow patterns spatially associated with platelet aggregates in murine colitis. | |
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MedLine Citation:
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PMID: 19645018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the normal murine mucosal plexus, blood flow is generally smooth and continuous. In inflammatory conditions, such as chemically-induced murine colitis, the mucosal plexus demonstrates markedly abnormal flow patterns. The inflamed mucosal plexus is associated with widely variable blood flow velocity as well as discontinuous and even bidirectional flow. To investigate the mechanisms responsible for these blood flow patterns, we used intravital microscopic examination of blood flow within the murine mucosal plexus during dextran sodium sulphate-and trinitrobenzenesulfonic acid-induced colitis. The blood flow patterns within the mucosal plexus demonstrated flow exclusion in 18% of the vessel segments (P < 0.01). Associated with these segmental exclusions was significant variation in neighboring flow velocities. Intravascular injection of fluorescent platelets demonstrated platelet incorporation into both fixed and rolling platelet aggregates. Rolling platelet aggregates (mean velocity 113 microm/sec; range, 14-186 microm/sec) were associated with reversible occlusions and flow variations within the mucosal plexus. Gene expression profiles of microdissected mucosal plexus demonstrated enhanced expression of genes for CCL3, CXCL1, CCL2, CXCL5, CCL7, CCL8, and Il-1b (P < 0.01), and decreased expression of CCL6 (P < 0.01). These results suggest that platelet aggregation, activated by the inflammatory mileau, contributes to the complex flow dynamics observed in acute murine colitis. |
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Authors:
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Lino F Miele; Aslihan Turhan; Grace S Lee; Miao Lin; Dino Ravnic; Akira Tsuda; Moritz A Konerding; Steven J Mentzer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Anatomical record (Hoboken, N.J. : 2007) Volume: 292 ISSN: 1932-8494 ISO Abbreviation: Anat Rec (Hoboken) Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-05 Completed Date: 2009-11-06 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101292775 Medline TA: Anat Rec (Hoboken) Country: United States |
Other Details:
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Languages: eng Pagination: 1143-53 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley-Liss, Inc. |
Affiliation:
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Laboratory of Adaptive and Regenerative Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Animals Blood Platelets / metabolism, physiology* Chemokines / genetics, metabolism Colitis / chemically induced, physiopathology* Dextran Sulfate / adverse effects Endothelium, Vascular / physiopathology Gene Expression Profiling Interleukin-1beta / genetics, metabolism Intestinal Mucosa / blood supply* Mice Mice, Inbred BALB C Mice, Inbred C57BL Microcirculation Platelet Aggregation* Regional Blood Flow Trinitrobenzenesulfonic Acid / adverse effects |
| Grant Support | |
ID/Acronym/Agency:
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HL054885/HL/NHLBI NIH HHS; HL070542/HL/NHLBI NIH HHS; HL074022/HL/NHLBI NIH HHS; HL47078/HL/NHLBI NIH HHS; HL75426/HL/NHLBI NIH HHS; R01 HL094567-01A1/HL/NHLBI NIH HHS; R01 HL094567-02/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Interleukin-1beta; 2508-19-2/Trinitrobenzenesulfonic Acid; 9042-14-2/Dextran Sulfate |
| Comments/Corrections | |
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