Document Detail


Blood flow patterns spatially associated with platelet aggregates in murine colitis.
MedLine Citation:
PMID:  19645018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the normal murine mucosal plexus, blood flow is generally smooth and continuous. In inflammatory conditions, such as chemically-induced murine colitis, the mucosal plexus demonstrates markedly abnormal flow patterns. The inflamed mucosal plexus is associated with widely variable blood flow velocity as well as discontinuous and even bidirectional flow. To investigate the mechanisms responsible for these blood flow patterns, we used intravital microscopic examination of blood flow within the murine mucosal plexus during dextran sodium sulphate-and trinitrobenzenesulfonic acid-induced colitis. The blood flow patterns within the mucosal plexus demonstrated flow exclusion in 18% of the vessel segments (P < 0.01). Associated with these segmental exclusions was significant variation in neighboring flow velocities. Intravascular injection of fluorescent platelets demonstrated platelet incorporation into both fixed and rolling platelet aggregates. Rolling platelet aggregates (mean velocity 113 microm/sec; range, 14-186 microm/sec) were associated with reversible occlusions and flow variations within the mucosal plexus. Gene expression profiles of microdissected mucosal plexus demonstrated enhanced expression of genes for CCL3, CXCL1, CCL2, CXCL5, CCL7, CCL8, and Il-1b (P < 0.01), and decreased expression of CCL6 (P < 0.01). These results suggest that platelet aggregation, activated by the inflammatory mileau, contributes to the complex flow dynamics observed in acute murine colitis.
Authors:
Lino F Miele; Aslihan Turhan; Grace S Lee; Miao Lin; Dino Ravnic; Akira Tsuda; Moritz A Konerding; Steven J Mentzer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  292     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-05     Completed Date:  2009-11-06     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1143-53     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Blood Platelets / metabolism,  physiology*
Chemokines / genetics,  metabolism
Colitis / chemically induced,  physiopathology*
Dextran Sulfate / adverse effects
Endothelium, Vascular / physiopathology
Gene Expression Profiling
Interleukin-1beta / genetics,  metabolism
Intestinal Mucosa / blood supply*
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microcirculation
Platelet Aggregation*
Regional Blood Flow
Trinitrobenzenesulfonic Acid / adverse effects
Grant Support
ID/Acronym/Agency:
HL054885/HL/NHLBI NIH HHS; HL070542/HL/NHLBI NIH HHS; HL074022/HL/NHLBI NIH HHS; HL47078/HL/NHLBI NIH HHS; HL75426/HL/NHLBI NIH HHS; R01 HL094567/HL/NHLBI NIH HHS; R01 HL094567-01A1/HL/NHLBI NIH HHS; R01 HL094567-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Chemokines; 0/Interleukin-1beta; 8T3HQG2ZC4/Trinitrobenzenesulfonic Acid; 9042-14-2/Dextran Sulfate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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