| Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems. | |
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MedLine Citation:
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PMID: 15665998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of colored microspheres to adequately evaluate blood flow changes under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. The aim of the present study was to validate the use of four different colors measuring four repeated blood flow changes in the same rat to assess the role of vasopressor systems in controlling arterial pressure (AP). Red (150,000), white (200,000), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 microg/kg, iv), 3) renin-angiotensin (losartan, 10 mg/kg, iv), and 4) sympathetic system blockade (hexamethonium, 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. The aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). In conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow. |
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Authors:
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K De Angelis; V M Gama; V A M Farah; M C Irigoyen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2005-01-18 |
Journal Detail:
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Title: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] Volume: 38 ISSN: 0100-879X ISO Abbreviation: Braz. J. Med. Biol. Res. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-24 Completed Date: 2005-06-27 Revised Date: 2008-08-16 |
Medline Journal Info:
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Nlm Unique ID: 8112917 Medline TA: Braz J Med Biol Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 119-25 Citation Subset: IM |
Affiliation:
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Unidade de Hipertensão, Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antihypertensive Agents / pharmacology* Arginine Vasopressin / analogs & derivatives*, pharmacology Blood Flow Velocity / drug effects* Blood Pressure / drug effects* Cardiac Output / drug effects Color Hexamethonium / pharmacology Losartan / pharmacology Male Microspheres* Rats Rats, Wistar Regional Blood Flow / drug effects Spectrophotometry, Atomic |
| Chemical | |
Reg. No./Substance:
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0/Antihypertensive Agents; 113-79-1/Arginine Vasopressin; 114798-26-4/Losartan; 60-26-4/Hexamethonium; 73168-24-8/vasopressin, 1-(1-mercaptocyclohexaneacetic acid)-2-(O- methyl-L-tyrosine)-8-L-arginine- |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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