Document Detail


Blood dendritic cell levels and phenotypic characteristics in relation to etiology of end-stage heart failure: implications for dilated cardiomyopathy.
MedLine Citation:
PMID:  18243370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Dysregulation of dendritic cell (DC) mediated immune responses towards auto-antigens, is considered an important feature in the maintenance of experimentally induced heart failure (HF). In order to evaluate the role of blood DCs in cardiomyopathies of different origins, we examined myeloid (mDC) and plasmacytoid (pDC) subset levels and maturation characteristics, according to HF severity and etiology in humans. METHODS: Absolute numbers of mDCs and pDCs in 12 New York Heart Association (NYHA) class-II, 28 NYHA class III-IV HF patients and 18 healthy controls, were studied by 4-colour whole blood flow cytometry. RESULTS: End-stage (NYHA III-IV) HF patients had comparable circulating DC subset levels to NYHA-II patients and controls. However, within the NYHA III-IV group total DC levels in patients with non-ischemic dilated cardiomyopathy (DCM) were higher (P<0.001) than in patients with coronary artery disease (CAD), hypertrophic cardiomyopathy (HCM) or other HF etiology. This was due to a significant increase of primarily mDCs (P<0.0001) and to a lesser extent of pDCs (P<0.05) in idiopathic DCM patients, independent of systolic or diastolic cardiac dysfunction. Maturation marker CD83 and lymphoid homing chemokine receptor CCR7 surface expression was enhanced only on mDCs, but not pDCs from DCM patients (P<0.05), compared to patients with CAD, HCM or other underlying cardiac pathophysiology. CONCLUSIONS: Total blood DC levels in end-stage HF are elevated in patients with DCM. Whole blood DC characterisation may lead to new insights into the pathophysiology of idiopathic DCM in humans.
Authors:
Petros Athanassopoulos; Aggie H M M Balk; Leonard M B Vaessen; Kadir Caliskan; Johanna J M Takkenberg; Willem Weimar; Ad J J C Bogers
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-02-19
Journal Detail:
Title:  International journal of cardiology     Volume:  131     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-22     Completed Date:  2009-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  246-56     Citation Subset:  IM    
Affiliation:
Department of Cardiothoracic Surgery, Erasmus MC, Rotterdam, The Netherlands. p.athanassopoulos@erasmusmc.nl
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Cardiomyopathy, Dilated / blood,  immunology*,  pathology*
Cross-Sectional Studies
Dendritic Cells / immunology,  pathology*
Female
Heart Failure / blood,  immunology*,  pathology*
Humans
Immunophenotyping*
Male
Middle Aged

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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