Document Detail

Blood-brain barrier controls carnitine level in the brain: a study on a model system with RBE4 cells.
MedLine Citation:
PMID:  10623637     Owner:  NLM     Status:  MEDLINE    
Transport of carnitine was studied with immortalized rat brain endothelial cells (RBE4), an in vitro model of the blood-brain barrier. The experiments on uptake and efflux through the luminal membrane excluded any involvement of choline and amino acids transporters, as well as that of glycoprotein P. Acetyl-, octanoylcarnitine, and betaine were without any effect; the only compound decreasing both processes was butyrobetaine. An exposure of the abluminal membrane resulted in a 40% inhibition of carnitine uptake by the substrates of neutral amino acid transporter L, while its efflux through the basolateral membrane, occurring in a form of free carnitine, was sensitive to SH group reagent, mersalyl, and was diminished by butyrobetaine. These features of carnitine transport did not fully correspond to the known characteristics of the proteins transporting carnitine in other tissues (OCTN2 and CT1); however, they did not exclude an involvement of a transporter belonging to the same superfamily. Moreover, such a protein in brain endothelium would fulfill a regulatory role in the transport of carnitine through the blood-brain barrier.
J E Mroczkowska; F S Roux; M J Naleçz; K A Naleçz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  267     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-08     Completed Date:  2000-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  433-7     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Nencki Institute of Experimental Biology, 3 Pasteur Street, Warsaw, 02-093, Poland.
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MeSH Terms
Acetylcarnitine / pharmacology
Betaine / pharmacology
Blood-Brain Barrier / physiology*
Brain / metabolism*
Carnitine / analogs & derivatives,  pharmacology
Cell Line
Cell Membrane / metabolism
Cerebrovascular Circulation
Endothelium, Vascular / drug effects,  metabolism*
Models, Biological
Reg. No./Substance:
107-43-7/Betaine; 14992-62-2/Acetylcarnitine; 3671-77-0/octanoylcarnitine; 541-15-1/Carnitine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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