Document Detail


Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens.
MedLine Citation:
PMID:  22608296     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
INTRODUCTION: Fifteen to sixty percent of cystic fibrosis patients harbor Aspergillus fumigatus (Af) in their airways (CF-AC) and some will develop allergic bronchopulmonary aspergillosis (CF-ABPA). Since basophils play a key role in allergy, we hypothesized that they would display alterations in CF-ABPA patients compared to CF-AC or patients without Af colonization (CF). METHODS: Using flow cytometry, we measured CD203c, CD63 and CD123 levels on basophils from CF-ABPA (N=11), CF-AC (N=14), and CF (N=12) patients before and after ex vivo stimulation with Af allergens. RESULTS: Baseline CD203c was increased in basophils from CF-ABPA compared to CF-AC and CF patients. Af extract and recombinant Aspf1 stimulated basophils from CF-ABPA patients to markedly upregulate CD203c, along with modest upregulation of CD63 and a CD123 downward trend. Plasma TARC/CCL17 at baseline and post-stimulation cell supernatant histamine levels were similar in the three groups. CONCLUSIONS: In CF-ABPA, blood basophils are primed and hyperresponsive to Af allergen stimulation.
Authors:
Yael Gernez; Colleen E Dunn; Cassie Everson; Erin Mitsunaga; Lakshmi Gudiputi; Karolina Krasinska; Zoe A Davies; Leonore A Herzenberg; Rabindra Tirouvanziam; Richard B Moss
Related Documents :
21327436 - Safety and effectiveness responses to etanercept for rheumatoid arthritis in japan: a s...
18192106 - Risk factors of venous thromboembolism in thai patients.
21344966 - Circumferential encasement of the spinal cord in a patient with rheumatoid arthritis.
8904186 - Recombinant factor viia (novoseven) in the treatment of internal bleeding in patients w...
20331856 - Near-infrared spectroscopy during stagnant ischemia estimates central venous oxygen sat...
18187976 - Auricular mucormycosis: a case report.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-16
Journal Detail:
Title:  Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society     Volume:  -     ISSN:  1873-5010     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128966     Medline TA:  J Cyst Fibros     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cimicifuga racemosa and its triterpene-saponins prevent the Metabolic Syndrome and deterioration of ...
Next Document:  Placebo, a historical perspective.