| Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens. | |
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MedLine Citation:
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PMID: 22608296 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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INTRODUCTION: Fifteen to sixty percent of cystic fibrosis patients harbor Aspergillus fumigatus (Af) in their airways (CF-AC) and some will develop allergic bronchopulmonary aspergillosis (CF-ABPA). Since basophils play a key role in allergy, we hypothesized that they would display alterations in CF-ABPA patients compared to CF-AC or patients without Af colonization (CF). METHODS: Using flow cytometry, we measured CD203c, CD63 and CD123 levels on basophils from CF-ABPA (N=11), CF-AC (N=14), and CF (N=12) patients before and after ex vivo stimulation with Af allergens. RESULTS: Baseline CD203c was increased in basophils from CF-ABPA compared to CF-AC and CF patients. Af extract and recombinant Aspf1 stimulated basophils from CF-ABPA patients to markedly upregulate CD203c, along with modest upregulation of CD63 and a CD123 downward trend. Plasma TARC/CCL17 at baseline and post-stimulation cell supernatant histamine levels were similar in the three groups. CONCLUSIONS: In CF-ABPA, blood basophils are primed and hyperresponsive to Af allergen stimulation. |
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Authors:
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Yael Gernez; Colleen E Dunn; Cassie Everson; Erin Mitsunaga; Lakshmi Gudiputi; Karolina Krasinska; Zoe A Davies; Leonore A Herzenberg; Rabindra Tirouvanziam; Richard B Moss |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-16 |
Journal Detail:
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Title: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society Volume: - ISSN: 1873-5010 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128966 Medline TA: J Cyst Fibros Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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