Document Detail


Blood pressure, blood flow, and oxygenation in the clipped kidney of chronic 2-kidney, 1-clip rats: effects of tempol and Angiotensin blockade.
MedLine Citation:
PMID:  20048199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiotensin II maintains renal cortical blood flow and renal oxygenation in the clipped kidney of early 2-kidney, 1-clip Goldblatt hypertensive (2K,1C) rats. The involvement of Ang II is believed to decline, whereas oxidative stress increases during the progression of 2K,1C hypertension. We investigated the hypothesis that the acute administration of drugs to inhibit reactive oxygen species (Tempol), angiotensin II type 1 receptors (candesartan), or angiotensin-converting enzyme (enalaprilat) lowers mean arterial pressure and increases kidney blood flow and oxygenation in the clipped kidney of chronic 2K,1C rats in contrast to sham controls. Twelve months after left renal artery clipping or sham, mean arterial pressure, renal cortical blood flow, and renal cortical and medullary oxygen tension were measured after acute administration of Tempol followed by enalaprilat or candesartan followed by enalaprilat. The mean arterial pressure of the 2K,1C rat was reduced by candesartan (-9%) and, more effectively, by Tempol (-35%). All of the applied treatments had similar blood pressure-lowering effects in sham rats (average: -21%). Only Tempol increased cortical blood flow (+35%) and cortical and medullary oxygen tensions (+17% and +94%, respectively) in clipped kidneys of 2K,1C rats. Administration of enalaprilat had no additional effect, except for a modest reduction in cortical blood flow in the clipped kidney of 2K,1C rats when coadministered with candesartan (-10%). In conclusion, acute administration of Tempol is more effective than candesartan in reducing the mean arterial blood pressure and improving renal blood perfusion and oxygenation in the clipped kidney of chronic 2K,1C rats.
Authors:
Fredrik Palm; Maristela Onozato; William J Welch; Christopher S Wilcox
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-01-04
Journal Detail:
Title:  Hypertension     Volume:  55     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-02-22     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-304     Citation Subset:  IM    
Affiliation:
Division of Nephrology and Hypertension, Hypertension, Kidney, and Vascular Center, and Angiogenesis Program of the Lombardi Cancer Center, Georgetown University, Washington, DC 20007, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Antihypertensive Agents / pharmacology
Benzimidazoles / pharmacology*
Blood Pressure / drug effects,  physiology
Cyclic N-Oxides / pharmacology*
Disease Models, Animal
Enalaprilat / pharmacology*
Glomerular Filtration Rate / drug effects
Hypertension, Renovascular / metabolism,  physiopathology*
Male
Organ Size
Oxygen Consumption / physiology*
Probability
Random Allocation
Rats
Rats, Sprague-Dawley
Renal Circulation / drug effects,  physiology
Spin Labels
Tetrazoles / pharmacology*
Vascular Resistance / drug effects
Grant Support
ID/Acronym/Agency:
DK-07183/DK/NIDDK NIH HHS; DK-077858/DK/NIDDK NIH HHS; DK-36079/DK/NIDDK NIH HHS; DK-49870/DK/NIDDK NIH HHS; HL-68686/HL/NHLBI NIH HHS; P01 HL068686-08/HL/NHLBI NIH HHS; R00 DK077858-03/DK/NIDDK NIH HHS; R01 DK049870-15/DK/NIDDK NIH HHS; R01 DK049870-17/DK/NIDDK NIH HHS; R01 DK072183-04/DK/NIDDK NIH HHS; R37 DK036079-23S1/DK/NIDDK NIH HHS; R37 DK036079-25/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Benzimidazoles; 0/Cyclic N-Oxides; 0/Spin Labels; 0/Tetrazoles; 139481-59-7/candesartan; 2226-96-2/tempol; 84680-54-6/Enalaprilat
Comments/Corrections

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