| Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota. | |
| | |
MedLine Citation:
|
PMID: 21445237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The presence of bacteria in the midgut of mosquitoes antagonizes infectious agents, such as Dengue and Plasmodium, acting as a negative factor in the vectorial competence of the mosquito. Therefore, knowledge of the molecular mechanisms involved in the control of midgut microbiota could help in the development of new tools to reduce transmission. We hypothesized that toxic reactive oxygen species (ROS) generated by epithelial cells control bacterial growth in the midgut of Aedes aegypti, the vector of Yellow fever and Dengue viruses. We show that ROS are continuously present in the midgut of sugar-fed (SF) mosquitoes and a blood-meal immediately decreased ROS through a mechanism involving heme-mediated activation of PKC. This event occurred in parallel with an expansion of gut bacteria. Treatment of sugar-fed mosquitoes with increased concentrations of heme led to a dose dependent decrease in ROS levels and a consequent increase in midgut endogenous bacteria. In addition, gene silencing of dual oxidase (Duox) reduced ROS levels and also increased gut flora. Using a model of bacterial oral infection in the gut, we show that the absence of ROS resulted in decreased mosquito resistance to infection, increased midgut epithelial damage, transcriptional modulation of immune-related genes and mortality. As heme is a pro-oxidant molecule released in large amounts upon hemoglobin degradation, oxidative killing of bacteria in the gut would represent a burden to the insect, thereby creating an extra oxidative challenge to the mosquito. We propose that a controlled decrease in ROS levels in the midgut of Aedes aegypti is an adaptation to compensate for the ingestion of heme. |
| | |
Authors:
|
Jose Henrique M Oliveira; Renata L S Gonçalves; Flavio A Lara; Felipe A Dias; Ana Caroline P Gandara; Rubem F S Menna-Barreto; Meredith C Edwards; Francisco R M Laurindo; Mário A C Silva-Neto; Marcos H F Sorgine; Pedro L Oliveira |
Related Documents
:
|
8810507 - In vivo and in vitro analysis of bordetella pertussis catalase and fe-superoxide dismut... 12218147 - Induction of cd8+ t lymphocytes by salmonella typhimurium is independent of salmonella ... 16557837 - Immunity in experimental salmonellosis i. protection induced by rough mutants of salmon... 7768607 - Interleukin-6-deficient mice are highly susceptible to listeria monocytogenes infection... 14660607 - Caveolin-1 knockdown by small interfering rna suppresses responses to the chemokine mon... 1937747 - Encapsulation of cryptococcus neoformans impairs antigen-specific t-cell responses. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-17 |
Journal Detail:
|
Title: PLoS pathogens Volume: 7 ISSN: 1553-7374 ISO Abbreviation: PLoS Pathog. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-29 Completed Date: 2011-07-18 Revised Date: 2011-07-27 |
Medline Journal Info:
|
Nlm Unique ID: 101238921 Medline TA: PLoS Pathog Country: United States |
Other Details:
|
Languages: eng Pagination: e1001320 Citation Subset: IM |
Affiliation:
|
Laboratório de Bioquímica de Artrópodes Hematófagos, Instituto de Bioquímica Médica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aedes
/
microbiology* Animals Heme / metabolism*, pharmacology Hemoglobins / metabolism*, pharmacology Humans Insect Proteins / metabolism* Oxidative Stress* Rabbits Reactive Oxygen Species / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
//Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
|
0/Hemoglobins; 0/Insect Proteins; 0/Reactive Oxygen Species; 14875-96-8/Heme |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Host iron withholding demands siderophore utilization for Candida glabrata to survive macrophage kil...
Next Document: Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.