Document Detail


Blood flow dynamics of one cardiac cycle and relationship to mechanotransduction and trabeculation during heart looping.
MedLine Citation:
PMID:  21239637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analyses of form-function relationships during heart looping are directly related to technological advances. Recent advances in four-dimensional optical coherence tomography (OCT) permit observations of cardiac dynamics at high-speed acquisition rates and high resolution. Real-time observation of the avian stage 13 looping heart reveals that interactions between the endocardial and myocardial compartments are more complex than previously depicted. Here we applied four-dimensional OCT to elucidate the relationships of the endocardium, myocardium, and cardiac jelly compartments in a single cardiac cycle during looping. Six cardiac levels along the longitudinal heart tube were each analyzed at 15 time points from diastole to systole. Using image analyses, the organization of mechanotransducing molecules, fibronectin, tenascin C, α-tubulin, and nonmuscle myosin II was correlated with specific cardiac regions defined by OCT data. Optical coherence microscopy helped to visualize details of cardiac architectural development in the embryonic mouse heart. Throughout the cardiac cycle, the endocardium was consistently oriented between the midline of the ventral floor of the foregut and the outer curvature of the myocardial wall, with multiple endocardial folds allowing high-volume capacities during filling. The cardiac area fractional shortening is much higher than previously published. The in vivo profile captured by OCT revealed an interaction of the looping heart with the extra-embryonic splanchnopleural membrane providing outside-in information. In summary, the combined dynamic and imaging data show the developing structural capacity to accommodate increasing flow and the mechanotransducing networks that organize to effectively facilitate formation of the trabeculated four-chambered heart.
Authors:
Barbara Garita; Michael W Jenkins; Mingda Han; Chao Zhou; Michael Vanauker; Andrew M Rollins; Michiko Watanabe; J G Fujimoto; Kersti K Linask
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-01-14
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  300     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-03     Completed Date:  2011-05-26     Revised Date:  2013-08-01    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H879-91     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, The Children’s Research Institute, University of South Florida and All Children’s Hospital, St. Petersburg, USA. klinask@health.usf.edu.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibronectins / physiology
Heart / embryology,  physiology*
Mechanotransduction, Cellular / physiology*
Mice
Myocardial Contraction / physiology
Myocardium / chemistry
Myosin Type II / physiology
Quail / physiology
Tenascin / physiology
Tomography, Optical Coherence
Tubulin / physiology
Grant Support
ID/Acronym/Agency:
C06 RR12463-01/RR/NCRR NIH HHS; HL083048/HL/NHLBI NIH HHS; HL096717/HL/NHLBI NIH HHS; R01 CA075289/CA/NCI NIH HHS; R01 EY011289/EY/NEI NIH HHS; R01 EY011289-26/EY/NEI NIH HHS; R01-CA075289-13/CA/NCI NIH HHS; T32EB007509/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Tenascin; 0/Tubulin; EC 3.6.1.-/Myosin Type II
Comments/Corrections

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