| Is the blood donated by habitual nut quid chewers suitable for use in transfusion? | |
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MedLine Citation:
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PMID: 20206834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Betel quid (BQ) chewing is a popular oral masticatory activity, and there are approximately 600 million BQ chewers worldwide. Although chewing BQ has been linked to the patho-genesis of oral cancer, leukoplakia, and oral submucous fibrosis. The question whether the mixed constituents present in areca nut, which may exert cytotoxic effects on red blood cells (RBCs), has never been addressed. METHODS: Heparinized blood specimens were obtained with informed consent from healthy laboratory personnel. RBCs were separated with the standard procedure and adjusted to 10% hematocrit with PBS. Various concentrations of areca nut extract (ANE; 100-800 microg/mL) were added to these RBC preparations and incubated at 37 degrees C for 4 hours. Two portions (0.4 mL each) of the incubated RBCs were then used for measuring osmotic deformability index and for observing RBC morphology with scanning electron microscopy. The remaining RBCs were used for determining membrane sulfhydryl groups and protein profiles by sodium dodecyl sulfate polyacrylamide gel electrophoresis. RESULTS: Blood incubated with various concentrations of ANE showed concentration-dependent decreases in osmotic deformability index and membrane sulfhydryl groups. Membrane protein profiles revealed a significant loss of the band 3 fraction, with the concomitant appearance of several new protein bands in the electropheretogram. Finally, drastic morphological changes of ANE-treated RBCs were observed. CONCLUSION: We suggest that to assure the quality of transfusion, the blood donated by a habitual BQ chewer should be used with caution because of its possible contamination with areca nut ingredients that may be cytotoxic to RBCs. |
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Authors:
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Kuang-Ping Peng; Jeng-Fong Chiou; Yen-Tsun Chen; Tsan-Zon Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the Formosan Medical Association = Taiwan yi zhi Volume: 109 ISSN: 0929-6646 ISO Abbreviation: J. Formos. Med. Assoc. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-03-08 Completed Date: 2010-04-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214933 Medline TA: J Formos Med Assoc Country: China (Republic : 1949- ) |
Other Details:
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Languages: eng Pagination: 106-12 Citation Subset: IM |
Copyright Information:
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(c) 2010 Formosan Medical Association & Elsevier. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Family Medicine, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Areca
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metabolism,
toxicity* Blood Donors* Blood Transfusion Electrophoresis, Polyacrylamide Gel Erythrocyte Deformability / drug effects* Erythrocytes / drug effects* Humans Mastication Membrane Proteins / metabolism Microscopy, Electron, Scanning Nuts / adverse effects, chemistry*, metabolism Plant Extracts / adverse effects, chemistry* Sodium Dodecyl Sulfate |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; 0/Plant Extracts; 151-21-3/Sodium Dodecyl Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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