Document Detail


Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation in bisphenol A-stimulated Chang liver cells.
MedLine Citation:
PMID:  18246544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dioscorea batatas Decne (DBD) is traditionally used to heal inflammatory disease as a folk medicine. It was reported that a glycoprotein (DBD glycoprotein) with a molecular weight of 30 kDa was isolated from DBD and consists of carbohydrate (83.75%) and protein (16.25%) moieties. The previous results showed that it has a strong scavenging activity against hydroxyl radicals without any pro-oxidant activity in the cell-free system. The purpose of the present study was to show whether or not the DBD glycoprotein inhibits cell proliferation-related signal transduction stimulated by bisphenol A (BPA, an environmental hormone) in Chang liver cells. The results in this study indicated that DBD glycoprotein (200 microg ml(-1)) has suppressive effects on abnormal cell viability, production of intracellular reactive oxygen species (ROS) and nitric oxide (NO) in BPA (50 microM)-induced Chang liver cells by blocking the phosphorylation of mitogen-activated protein kinase (MAPK) and activating protein-1 (AP-1) activity. In addition, DBD glycoprotein (200 microg ml(-1)) normalized the activity of catalase (CAT) and glutathione peroxidase (GPx). Consequently, DBD glycoprotein inhibits the expression of proliferating cell nuclear antigen (PCNA, cell proliferation maker) stimulated by BPA. Therefore, it is speculated that DBD glycoprotein protects against carcinogenic events caused by BPA in Chang liver cells.
Authors:
Phil-Sun Oh; Kye-Taek Lim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied toxicology : JAT     Volume:  28     ISSN:  0260-437X     ISO Abbreviation:  J Appl Toxicol     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-10-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109495     Medline TA:  J Appl Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  749-58     Citation Subset:  IM    
Copyright Information:
Copyright 2008 John Wiley & Sons, Ltd.
Affiliation:
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju, 500-757, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Dioscorea / chemistry*
Free Radical Scavengers / pharmacology*
Glycoproteins / pharmacology*
Hepatocytes / drug effects,  metabolism*
Humans
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogens / toxicity
Nitric Oxide / metabolism
Oxidative Stress / drug effects
Phenols / pharmacology*
Phosphorylation
Proliferating Cell Nuclear Antigen / pharmacology
Reactive Oxygen Species / antagonists & inhibitors*,  metabolism*
Signal Transduction / drug effects
Superoxides / metabolism
Transcription Factor AP-1 / metabolism
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Glycoproteins; 0/Mitogens; 0/Phenols; 0/Proliferating Cell Nuclear Antigen; 0/Reactive Oxygen Species; 0/Transcription Factor AP-1; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; 80-05-7/bisphenol A; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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