Document Detail


Blocking endogenous glypican-3 expression releases Hep 3B cells from G1 arrest.
MedLine Citation:
PMID:  12872992     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glypican-3 (GPC3) encodes a cell-surface heparan-sulfate proteoglycan mutated in type 1 Simpson-Golabi-Behmel syndrome (SGBS1), an X-linked overgrowth syndrome. The phenotype of SGBS1 patients and of GPC3 knockout mice suggests that GPC3 plays a negative role in cell proliferation, and an apoptosis-inducing role in specific tissues. Ectopic expression of GPC3 in some cell lines has supported the idea that GPC3 inhibits cell growth. Here we report that blocking endogenous GPC3 expression with an antisense transcript promotes the growth of Hep G2 and Hep 3B hepatoma cell lines. Moreover, antisense inhibition releases Hep 3B cells from cell cycle arrest. Hence, our data further support the notion that GPC3 is an inhibitor of cell proliferation and demonstrate that it modulates cell cycle progression.
Authors:
Mohammad Farooq; Sun Young Hwang; Mi Kyung Park; Jung-Chul Kim; Moon Kyu Kim; Young Kwan Sung
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  15     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-22     Completed Date:  2004-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  356-60     Citation Subset:  IM    
Affiliation:
Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular
Cell Division / genetics*
Cell Line
G1 Phase
Glypicans
Heparan Sulfate Proteoglycans / genetics,  metabolism,  physiology*
Humans
Lipids / pharmacology
Mice
Oligonucleotides, Antisense / pharmacology
Rats
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Glypicans; 0/Heparan Sulfate Proteoglycans; 0/Lipids; 0/Lipofectamine; 0/Oligonucleotides, Antisense

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