Document Detail

Blocking antibodies inhibit complete African swine fever virus neutralization.
MedLine Citation:
PMID:  9213385     Owner:  NLM     Status:  MEDLINE    
A persistent non-neutralized African swine fever virus (ASFV) fraction is found with most convalescent swine sera in in vitro neutralization assays. To study this phenomenon, antisera from convalescent pigs infected with different virus isolates and showing complete or incomplete virus neutralization were used. Different experiments determined that incomplete neutralization of ASFV is caused neither by virus aggregation, nor low affinity or stability of virus-antibody complexes. Additionally, attempts to purify antigenic escape mutant viruses from the persistent fraction was also unsuccessful. Nevertheless, competition experiments between sera demonstrated that antibodies present in sera showing persistent fraction inhibited the complete neutralization mediated by antibodies present in sera which neutralize 100% of virus infectivity. These results suggest that induction of blocking antibodies during ASFV infection could represent the main cause for the persistent surviving virus fraction observed in neutralization assays and could also explain the persistent infections observed in some convalescent pigs.
P Gómez-Puertas; J M Escribano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  49     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-08-13     Completed Date:  1997-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  115-22     Citation Subset:  IM    
Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain.
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MeSH Terms
African Swine Fever / etiology,  immunology*,  virology
African Swine Fever Virus / immunology*
Antibodies, Blocking / physiology*
Antibodies, Viral / physiology*
Antigens, Viral / chemistry,  immunology
Cercopithecus aethiops
Neutralization Tests
Vero Cells
Reg. No./Substance:
0/Antibodies, Blocking; 0/Antibodies, Viral; 0/Antigens, Viral

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