Document Detail

Blocking Daxx trafficking attenuates neuronal cell death following ischemia/reperfusion in rat hippocampus CA1 region.
MedLine Citation:
PMID:  21843499     Owner:  NLM     Status:  Publisher    
Previous studies have shown that the death-associated protein (Daxx) shuttles between nucleus and cytoplasm under ischemic stress, and the subcellular localization of Daxx plays an important role in ischemic neuron death. In this study, by blocking the Daxx trafficking, the rat hippocampus CA1 neurons were protected against cerebral ischemia/reperfusion, and the molecular mechanism underlying this neuroprotection was studied. We found that pretreatment of SP600125, an inhibitor of c-Jun N-terminal kinase (JNK), or an anti-oxidant, N-acetylcysteine (NAC), could not only prevent Daxx from trafficking but also increase the number of the surviving CA1 pyramidal cells of hippocampus at 5days of reperfusion. Furthermore, knock-down of endogenous Daxx exerted similar neuroprotective effect during ischemia/reperfusion. We found the treatment of SP600125 or NAC could decrease the activation of Ask1 during ischemia/reperfusion and suppress the assembly of the Fas·Daxx·Ask1 signaling module, and in succession inhibit JNK activation and c-Jun phosphorylation. This study provides the Daxx trafficking as a new potential therapeutic target for ischemic brain injury.
Yu-Lan Niu; Chong Li; Guang-Yi Zhang
Related Documents :
382759 - A cytoarchitectonic scheme for the spinal cord of the domestic fowl, gallus gallus dome...
10634699 - Location of the phrenic nucleus in the human spinal cord.
18282609 - Morphological features and responses to ampa receptor-mediated excitotoxicity of mouse ...
2907119 - Release of immunoreactive somatostatin in the spinal dorsal horn of the cat.
16902759 - The calcium binding proteins calbindin, parvalbumin, and calretinin have specific patte...
22177179 - Corpus cavernosum electromyography revisited: defining the origin of the signal.
16756389 - Dynamic encoding of natural luminance sequences by lgn bursts.
530499 - On a possible neural mechanism underlying a finding by evarts and fromm.
9450529 - Immunocytochemical demonstration of locustatachykinin-related peptides in the central c...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-6
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  -     ISSN:  1096-0384     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Research Center for Biochemistry and Molecular Biology, Jiangsu Key Laboratory of Brain Disease Bioinformation, Xuzhou Medical College, Xuzhou 221002, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Substituted tryptophans at amyloid-?(1-40) residues 19 and 20 experience different environments afte...
Next Document:  Free cholesterol accumulation impairs antioxidant activities and aggravates apoptotic cell death in ...