Document Detail

Blockage of volume-activated chloride channels inhibits migration of nasopharyngeal carcinoma cells.
MedLine Citation:
PMID:  17495465     Owner:  NLM     Status:  MEDLINE    
Cell migration is crucial for tumor metastasis. Membrane ion channels may play a major role in tumor cell migration because the cells must undergo changes in shape and volume during migration. In the present study, we used the transwell migration assay, an in vitro model for cell migration, and the patch-clamp technique to investigate the role of the volume-activated Cl(-) current (I(cl,vol)) in the regulation of the migration of nasopharyngeal carcinoma CNE-2Z cells. 5-Nitro-2- (3-phenylpropylamino) benzoic acid (NPPB) inhibited the I(cl,vol) and the migration of CNE-2Z cells with almost identical dose-dependent pattern (IC(50) of 98.1 microM and 97.7 microM for I(cl,vol) and cell migration, respectively). Extracellular adenosine triphosphate (ATP) also showed similar dose-dependent inhibitory effects on the currents and migration (IC(50) of 1.07mM, and 1.11mM for I(cl,vol) and cell migration, respectively). Hypotonic treatments, which activated I(cl,vol), increased cell migration. Exposure to hypertonic solutions, which was shown to suppress I(cl,vol), inhibited cell migration. Replacement of Cl(-) with gluconate, which is relatively chloride channel-impermeable, impaired cell migration, whereas substitution of Cl(-) by I(-) and Br(-), the chloride channel-permeable ions, did not significantly affect cell migration. Analysis of the effects of all the above treatments on I(cl,vol) and cell migration indicated that the inhibition of migration was positively correlated with the blockage of I(cl,vol), with a correlation coefficient (r) of 0.97, suggesting a functional relationship between I(cl,vol) and cell migration. These data suggest that the volume-activated Cl(-) channels are involved in cell migration.
Jianwen Mao; Liwei Wang; Aihui Fan; Jianhong Wang; Bin Xu; Tim J C Jacob; Lixin Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  19     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2007  
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-07-27     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  249-58     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 S. Karger AG, Basel.
Institute of Basic Medical Sciences, Guangdong Pharmaceutical University, PR China.
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MeSH Terms
Cell Differentiation
Cell Movement / drug effects*
Cell Size
Chloride Channels / antagonists & inhibitors*,  metabolism
Nasopharyngeal Neoplasms / pathology*
Tetrodotoxin / pharmacology
Verapamil / pharmacology
Grant Support
056909/299/Z//Wellcome Trust
Reg. No./Substance:
0/Chloride Channels; 4368-28-9/Tetrodotoxin; 52-53-9/Verapamil

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