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Blockade of the Nuclear Factor Kappa B Pathway Prolonged Islet Allograft Survival.
MedLine Citation:
PMID:  22236081     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Nuclear factor kappa B (NF-κB) pathway is known for its important role in the upregulation of various inflammatory mediators and its "switch regulator" functionality for transcription factor gene networks which control cytokine-induced β-cell dysfunction and death. In this study, the islets were divided into the control group, Ad-green fluorescent protein, and the adenovirus transfected with inhibitor kappa B group. The proliferation index of peripheral blood mononuclear cells and the islets apoptosis index were examined after mixed lymphocyte-islet reaction with inverted fluorescence microscopy. Moreover, mRNA expression of inflammatory cytokines was measured by reverse transcription polymerase chain reaction. The islet graft survival time in diabetic rats, insulin in grafts, and cytokine concentrations in the supernatant were determined by immunohistochemistry and enzyme-linked immunosorbent assay. We found that blocking of NF-κB activation in β-cells significantly downregulated inflammatory chemokine production by islets cells in vitro and in vivo, inhibited T-cell recruitment into the pancreatic islets, inhibited β-cell dysfunction, and effectively prolonged the survival time of islet grafts. The results presented in this work highlight a novel mechanism of blocking NF-κB activation in β-cells for the treatment of islet cell transplantation.
Authors:
Xiaoming Ding; Xiaohong Wang; Wujun Xue; Xiaohui Tian; Yang Li; Fengmei Jiao; Xinshun Feng; Jin Zheng
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-12
Journal Detail:
Title:  Artificial organs     Volume:  -     ISSN:  1525-1594     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012, Copyright the Authors. Artificial Organs © 2012, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Affiliation:
Department of Renal Transplant, Center of Nephropathy, the First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an City, Shaanxi Province, China.
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