Document Detail


Block of c-Fos and JunB expression by antisense oligonucleotides inhibits light-induced phase shifts of the mammalian circadian clock.
MedLine Citation:
PMID:  7773436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Light-induced phase shifts of circadian rhythmic locomotor activity are associated with the expression of c-Jun, JunB, c-Fos and FosB transcription factors in the rat suprachiasmatic nucleus, as shown in the present study. In order to explore the importance of c-Fos and JunB, the predominantly expressed AP-1 proteins for the phase-shifting effects of light, we blocked the expression of c-Fos and JunB in the suprachiasmatic nucleus of male rats, housed under constant darkness, by intracerebroventricular application of 2 microliters of 1 mM antisense phosphorothioate oligodeoxynucleotides (ASO) specifically directed against c-fos and junB mRNA. A light pulse (300 lux for 1 h) at circadian time 15 induced a significant phase shift (by 125 +/- 15 min) of the circadian locomotor activity rhythm, whereas application of ASO 6 h before the light pulse completely prevented this phase shift. Application of control nonsense oligodeoxynucleotides had no effect. ASO strongly reduced the light-induced expression of c-Fos and JunB proteins. In contrast, light pulses with or without the control nonsense oligodeoxynucleotides evoked strong nuclear c-Fos and JunB immunoreactivity in the rat suprachiasmatic nucleus. These results demonstrate for the first time that inducible transcription factors such as c-Fos and JunB are an essential part of fundamental biological processes in the adult mammalian nervous system, e.g. of light-induced phase shifts of the circadian pacemaker.
Authors:
F Wollnik; W Brysch; E Uhlmann; F Gillardon; R Bravo; M Zimmermann; K H Schlingensiepen; T Herdegen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The European journal of neuroscience     Volume:  7     ISSN:  0953-816X     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-07-13     Completed Date:  1995-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  388-93     Citation Subset:  IM    
Affiliation:
Faculty of Biology, University of Konstanz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Circadian Rhythm / physiology*,  radiation effects*
Injections, Intraventricular
Light*
Male
Molecular Sequence Data
Motor Activity / physiology*,  radiation effects
Oligonucleotides, Antisense / genetics,  pharmacology
Proto-Oncogene Proteins c-fos / antagonists & inhibitors,  physiology*
Proto-Oncogene Proteins c-jun / antagonists & inhibitors,  physiology*
Rats
Suprachiasmatic Nucleus / physiology
Chemical
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun

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