Document Detail


Bleb morphology and histology in a rabbit model of glaucoma filtration surgery using Ozurdex® or mitomycin-C.
MedLine Citation:
PMID:  22509101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To determine the effect of a sustained-release dexamethasone implant (Ozurdex(®)) on wound healing after glaucoma filtration surgery in a rabbit model.
METHODS: Twelve new zealand white rabbits were divided into three groups: filtration surgery with intraoperative subconjunctival implantation of Ozurdex(®) (n=6), filtration surgery with intraoperative topical application of mitomycin-C (MMC; n=6), and filtration surgery with intraoperative topical application of balanced salt solution (BSS; n=12). A standard scale was used to grade bleb vascularity at three and six weeks after the initial operation. Bleb survival was also recorded for comparison between the three groups. Histologic analysis was performed with attention to cellularity and collagen deposition.
RESULTS: MMC-treated blebs demonstrated decreased numbers of goblet cells compared to all other groups. Blebs treated with Ozurdex(®) maintained a near normal number of goblet cells with modest collagen deposition. The control eyes treated with only BSS had significant collagen deposition and increased cellularity compared to both the Ozurdex(®) and MMC groups. Bleb vascularity was not significantly different among groups at the three and six week post-operative evaluations. MMC-treated and Ozurdex(®)-treated blebs had significantly prolonged bleb survival compared to blebs treated with only BSS. In addition, MMC-treated blebs had significantly longer survival compared to Ozurdex(®)-treated blebs.
CONCLUSIONS: The results of this study support the utility of extended-release dexamethasone (Ozurdex(®)) as a wound modulating agent in a rabbit model of filtration surgery. Further animal and human studies are needed to better characterize a possible role for Ozurdex(®) in filtration surgery.
Authors:
Jeffrey R SooHoo; Leonard K Seibold; Ashley E Laing; Malik Y Kahook
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-26
Journal Detail:
Title:  Molecular vision     Volume:  18     ISSN:  1090-0535     ISO Abbreviation:  Mol. Vis.     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-17     Completed Date:  2012-07-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9605351     Medline TA:  Mol Vis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  714-9     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Colorado Denver, Aurora, CO 80045, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anterior Chamber / drug effects*,  pathology,  surgery
Collagen / antagonists & inhibitors,  biosynthesis
Conjunctiva / drug effects*,  pathology,  surgery
Delayed-Action Preparations
Dexamethasone / administration & dosage,  therapeutic use*
Filtering Surgery
Glaucoma / drug therapy*,  pathology,  surgery
Goblet Cells / drug effects,  pathology
Humans
Intraocular Pressure
Mitomycin / administration & dosage,  therapeutic use
Rabbits
Wound Healing / drug effects
Chemical
Reg. No./Substance:
0/Delayed-Action Preparations; 50-02-2/Dexamethasone; 50-07-7/Mitomycin; 9007-34-5/Collagen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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