Document Detail


Blastomere removal from cleavage-stage mouse embryos alters steroid metabolism during pregnancy.
MedLine Citation:
PMID:  22517623     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Preimplantation genetic diagnosis (PGD) is a genetic screening of embryos conceived with assisted reproduction technologies (ART). A single blastomere from an early-stage embryo is removed and molecular analyses follow to identify embryos carrying genetic defects. PGD is considered highly successful for detecting genetic anomalies, but the effects of blastomere biopsy on fetal development are understudied. We aimed to determine whether single blastomere removal affects steroid homeostasis in the maternal-placental-fetal unit during mouse pregnancy. Embryos generated by in vitro fertilization (IVF) were biopsied at the four-cell stage, cultured to morula/early blastocyst, and transplanted into the oviducts of surrogate mothers. Nonbiopsied embryos from the same IVF cohorts served as controls. Cesarean section was performed at term, and maternal and fetal tissues were collected. Embryo biopsy affected the levels of steroids (estradiol, estrone, and progesterone) in fetal and placental compartments but not in maternal tissues. Steroidogenic enzyme activities (3beta-hydroxysteroid dehydrogenase, cytochrome P450 17alpha-hydroxylase, and cytochrome P450 19) were unaffected but decreased activities of steroid clearance enzymes (uridine diphosphate-glucuronosyltransferase and sulfotransferase) were observed in placentas and fetal livers. Although maternal body, ovarian, and placental weights did not differ, the weights of fetuses derived from biopsied embryos were lower than those of their nonbiopsied counterparts. The data demonstrate that blastomere biopsy deregulates steroid metabolism during pregnancy. This may have profound effects on several aspects of fetal development, of which low birth weight is only one. If a similar phenomenon occurs in humans, it may explain low birth weights associated with PGD/ART and provide a plausible target for improving PGD outcomes.
Authors:
Atsushi Sugawara; Brittany Sato; Elise Bal; Abby C Collier; Monika A Ward
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-05
Journal Detail:
Title:  Biology of reproduction     Volume:  87     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-06     Completed Date:  2012-11-30     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4, 1-9     Citation Subset:  IM    
Affiliation:
Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / metabolism
Animals
Aromatase / metabolism
Birth Weight
Blastomeres / cytology*,  metabolism*
Cell Separation
Cleavage Stage, Ovum / cytology*,  metabolism*
Female
Fertilization in Vitro
Fetal Development
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Models, Animal
Pregnancy
Preimplantation Diagnosis / adverse effects*,  methods
Steroid 17-alpha-Hydroxylase / metabolism
Steroids / metabolism*
Grant Support
ID/Acronym/Agency:
HD058059/HD/NICHD NIH HHS; RR024206/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Steroids; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.14.1/Aromatase; EC 1.14.99.9/Steroid 17-alpha-Hydroxylase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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