| Blackcurrant proanthocyanidins augment IFN-gamma-induced suppression of IL-4 stimulated CCL26 secretion in alveolar epithelial cells. | |
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MedLine Citation:
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PMID: 20229526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epidemiological studies reveal that fruit consumption reduces the prevalence of airway inflammation and childhood asthma. In particular, blackcurrant polyphenolic extracts have been shown to alleviate lung inflammation. Since IL-4-stimulated eotaxin-3 (CCL26) secretion is a major factor in the continuous eosinophil recruitment observed in atopic asthma, our focus was to evaluate the effectiveness of blackcurrant polyphenolic compounds on CCL26 secretion in human alveolar epithelial cells. Our results indicate that a proanthocyanin-enriched blackcurrant extract (BC-P), but not anthocyanin-enriched blackcurrant extract suppressed both IL-4- and IL-13-stimulated CCL26 secretion in a dose-dependent manner. Furthermore pre-incubation of cells with BC-P caused a time-dependent suppression of IL-4-stimulated CCL26 secretion. Moreover, epigallocatechin (EGC), and to a lesser extent epicatechin, metabolites identified in the proanthocyanidin extract, suppressed IL-4-stimulated CCL26 secretion. EGC was also effective at reducing the cellular phosphorylated STAT-6/STAT-6 ratio. Furthermore, both BC-P and purified EGC potentiated the ability of IFN-gamma to suppress IL-4-stimulated CCL26 secretion. The progression of an allergic immune response is complex, identifying plant compounds that target specific cellular events and complement the body's own immune actions is important for the development of functional foods. Our findings support the potential for blackcurrant polyphenolic compounds to reduce eosinophil recruitment and alleviate eosinophilic-driven airway inflammation. |
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Authors:
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Suzanne M Hurst; Tony K McGhie; Janine M Cooney; Dwayne J Jensen; Elaine M Gould; Kirsty A Lyall; Roger D Hurst |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular nutrition & food research Volume: 54 Suppl 2 ISSN: 1613-4133 ISO Abbreviation: Mol Nutr Food Res Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-12-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101231818 Medline TA: Mol Nutr Food Res Country: Germany |
Other Details:
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Languages: eng Pagination: S159-70 Citation Subset: IM |
Affiliation:
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Food Innovation portfolio, The Plant and Food Research Institute of New Zealand Ltd., Palmerston North, New Zealand. roger.hurst@plantandfood.co.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents, Non-Steroidal
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analysis,
chemistry,
pharmacology Asthma / prevention & control Catechin / analogs & derivatives, pharmacology Cell Line Chemokines, CC / genetics, metabolism, secretion* Epithelial Cells / drug effects, metabolism, secretion Fruit / chemistry Gene Expression Regulation / drug effects Humans Interferon-gamma, Recombinant / agonists, pharmacology* Interleukin-13 / antagonists & inhibitors, pharmacology Interleukin-4 / antagonists & inhibitors, pharmacology* Osmolar Concentration Phosphorylation / drug effects Plant Extracts / chemistry, pharmacology* Proanthocyanidins / analysis, chemistry, pharmacology* Pulmonary Alveoli / drug effects*, metabolism, secretion RNA, Messenger / metabolism Recombinant Proteins / pharmacology Ribes / chemistry* STAT6 Transcription Factor / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/CCL26 protein, human; 0/Chemokines, CC; 0/IL4 protein, human; 0/Interferon-gamma, Recombinant; 0/Interleukin-13; 0/Plant Extracts; 0/Proanthocyanidins; 0/RNA, Messenger; 0/Recombinant Proteins; 0/STAT6 Transcription Factor; 0/STAT6 protein, human; 154-23-4/Catechin; 1617-55-6/gallocatechol; 207137-56-2/Interleukin-4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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